微晶体电子衍射（MicroED）的进展与应用。
Advances and applications of microcrystal electron diffraction (MicroED).
机构信息
Chemical Engineering, School for Engineering of Matter, Transport and Energy, Arizona State University, Tempe, AZ 85287, USA; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Chemical Engineering, School for Engineering of Matter, Transport and Energy, Arizona State University, Tempe, AZ 85287, USA; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, USA.
出版信息
Curr Opin Struct Biol. 2024 Feb;84:102741. doi: 10.1016/j.sbi.2023.102741. Epub 2023 Dec 11.
Microcrystal electron diffraction, commonly referred to as MicroED, has become a powerful tool for high-resolution structure determination. The method makes use of cryogenic transmission electron microscopes to collect electron diffraction data from crystals that are several orders of magnitude smaller than those used by other conventional diffraction techniques. MicroED has been used on a variety of samples including soluble proteins, membrane proteins, small organic molecules, and materials. Here we will review the MicroED method and highlight recent advancements to the methodology, as well as describe applications of MicroED within the fields of structural biology and chemical crystallography.
微晶电子衍射，通常称为 MicroED，已成为高分辨率结构测定的有力工具。该方法利用低温透射电子显微镜从比其他常规衍射技术小几个数量级的晶体中收集电子衍射数据。MicroED 已应用于多种样品，包括可溶性蛋白质、膜蛋白、小分子和材料。在这里，我们将回顾 MicroED 方法，并重点介绍该方法的最新进展，以及描述 MicroED 在结构生物学和化学晶体学领域的应用。
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