Oncology Department No. 2, Linyi People's Hospital, Linyi City, Shandong Province, 276000, China.
Radiotherapy Department, Linyi People's Hospital, Linyi City, Shandong Province, 276000, China.
Asian Pac J Trop Med. 2016 Feb;9(2):180-3. doi: 10.1016/j.apjtm.2016.01.010. Epub 2016 Jan 11.
To study the inhibiting effect of Endostar combined with ginsenoside Rg3 on breast cancer tumor growth in tumor-bearing mice.
Female mice were selected as experimental animals, and breast cancer tumor-bearing mouse models were established and then divided into groups A, B, C and D that respectively received saline, recombinant human endostatin, ginsenosides Rg3 and recombinant human endostatin combined with Rg3 intervention; 7 d, 14 d and 21 d after intervention, tumor tissue volume was measured; 21 d after intervention, mice were killed, tumor tissue was collected, and mRNA contents of angiogenesis molecules, invasion molecules, autophagy marker molecules and autophagy signaling pathway molecules were detected.
At 7 d, 14 d and 21 d after intervention, tumor tissue volume of groups B, C and D was lower than that of group A, and tumor tissue volume of group D was lower than that of groups B and C; mRNA contents of VEGFA, VEGFB, VEGFC, MMP2, MMP9, p62, mTOR, PI3K, Akt, JNK and Beclin-1 in tumor tissue of groups B, C and D were significantly lower than those of group A, and LC3-II/LC3-I was significantly higher than that of group A; mRNA contents of VEGFA, VEGFB, VEGFC, MMP2, MMP9, p62, mTOR, PI3K, Akt, JNK and Beclin-1 in tumor tissue of group D were significantly lower than those of groups B and C, and LC3-II/LC3-I was higher than that of groups B and C.
Endostar combined with ginsenoside Rg3 has stronger inhibiting effect on breast cancer tumor growth in tumor-bearing mice than single drug, and it can inhibit angiogenesis and cell invasion, and enhance cell autophagy.
研究恩度联合人参皂甙 Rg3 对荷瘤小鼠乳腺癌肿瘤生长的抑制作用。
选用雌性小鼠作为实验动物，建立乳腺癌荷瘤小鼠模型，然后分为 A、B、C、D 组，分别给予生理盐水、重组人血管内皮抑制素、人参皂甙 Rg3、重组人血管内皮抑制素联合 Rg3 干预；干预后 7、14、21 d 测量肿瘤组织体积；干预 21 d 后处死小鼠，收集肿瘤组织，检测血管生成分子、侵袭分子、自噬标记分子和自噬信号通路分子的 mRNA 含量。
干预后 7、14、21 d，B、C、D 组肿瘤组织体积均低于 A 组，D 组低于 B、C 组；B、C、D 组肿瘤组织中 VEGFA、VEGFB、VEGFC、MMP2、MMP9、p62、mTOR、PI3K、Akt、JNK 和 Beclin-1 的 mRNA 含量均明显低于 A 组，LC3-II/LC3-I 明显高于 A 组；D 组肿瘤组织中 VEGFA、VEGFB、VEGFC、MMP2、MMP9、p62、mTOR、PI3K、Akt、JNK 和 Beclin-1 的 mRNA 含量均明显低于 B、C 组，LC3-II/LC3-I 高于 B、C 组。
恩度联合人参皂甙 Rg3 对荷瘤小鼠乳腺癌肿瘤生长的抑制作用强于单药，能抑制血管生成和细胞侵袭，增强细胞自噬。
