Liu Chun-Yen, Matsusaki Michiya, Akashi Mitsuru
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Graduate School of Frontier of Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
Biochem Biophys Res Commun. 2016 Mar 25;472(1):131-6. doi: 10.1016/j.bbrc.2016.02.080. Epub 2016 Feb 23.
Engineering three-dimensional (3D) vascularized constructs remains a central challenge because capillary network structures are important for sufficient oxygen and nutrient exchange to sustain the viability of engineered constructs. However, construction of 3D-tissues at single cell level has yet to be reported. Previously, we established a collagen coating method for fabricating a micrometer-sized collagen matrix on cell surfaces to control cell distance or cell densities inside tissues. In this study, a simple fabrication method is presented for constructing vascular networks in 3D-tissues over micrometer-sized or even millimeter-sized with controlled cell densities. From the results, well vascularized 3D network structures can be observed with a fluorescence label method mixing collagen coated cells and endothelia cells, indicating that constructed ECM rich tissues have the potential for vascularization, which opens up the possibility for various applications in pharmaceutical or tissue engineering fields.
构建三维（3D）血管化组织仍然是一个核心挑战，因为毛细血管网络结构对于充足的氧气和营养物质交换以维持工程组织的活力至关重要。然而，在单细胞水平构建3D组织尚未见报道。此前，我们建立了一种胶原包被方法，用于在细胞表面制造微米级胶原基质，以控制组织内的细胞间距或细胞密度。在本研究中，提出了一种简单的制造方法，用于在微米级甚至毫米级的3D组织中构建具有可控细胞密度的血管网络。结果表明，通过将胶原包被细胞与内皮细胞混合的荧光标记方法，可以观察到血管化良好的3D网络结构，这表明构建的富含细胞外基质的组织具有血管化潜力，为制药或组织工程领域的各种应用开辟了可能性。
