Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
Department of Gastroenterology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, China.
Cell Tissue Res. 2021 Nov;386(2):215-226. doi: 10.1007/s00441-020-03397-1. Epub 2021 Aug 14.
Recently, the introduction of surface acoustic wave (SAW) technique for microfluidics has drawn a lot of attention. The pattern and mutual communication in cell layers, tissues, and organs play a critical role in tissue homeostasis and regeneration and may contribute to disease occurrence and progression. Tissue engineering aims to repair and regenerate damaged organs, depending on biomimetic scaffolds and advanced fabrication technology. However, traditional bioengineering synthesis approaches are time-consuming, heterogeneous, and unmanageable. It is hard to pattern cells in scaffolds effectively with no impact on cell viability and function. Here, we summarize a biocompatible, easily available, label-free, and non-invasive tool, surface acoustic wave (SAW) technique, which is getting a lot of attention in tissue engineering. SAW technique can realize accurate sorting, manipulation, and cells' pattern and rapid formation of spheroids. By integrating several SAW devices onto lab-on-a-chip platforms, tissue engineering lab-on-a-chip system was proposed. To the best of our knowledge, this is the first report to summarize the application of this novel technique in the field of tissue engineering.
最近，表面声波（SAW）技术在微流控领域的引入引起了广泛关注。细胞层、组织和器官中的模式和相互交流在组织稳态和再生中起着关键作用，并可能导致疾病的发生和发展。组织工程旨在修复和再生受损的器官，这依赖于仿生支架和先进的制造技术。然而，传统的生物工程合成方法耗时、异质且难以管理。在不影响细胞活力和功能的情况下，有效地在支架中对细胞进行图案化是很困难的。在这里，我们总结了一种生物相容性好、易于获得、无标记且非侵入性的工具，即表面声波（SAW）技术，该技术在组织工程中受到了广泛关注。SAW 技术可实现精确的分选、操作和细胞图案化以及快速形成球体。通过将几个 SAW 器件集成到芯片实验室平台上，提出了组织工程芯片实验室系统。据我们所知，这是第一篇总结该新技术在组织工程领域应用的报告。
