Simsek Nejdet, Koc Akif, Karadeniz Ali, Yildirim Mehmet Erol, Celik Hüseyin Tuğrul, Sari Erhan, Kara Adem
Department of Histology and Embryology, Faculty of Medicine, Balıkesir University, Balıkesir, Turkey.
Department of Urology, Faculty of Medicine, Balıkesir University, Balıkesir, Turkey.
Acta Histochem. 2016 Apr;118(3):263-70. doi: 10.1016/j.acthis.2016.02.002. Epub 2016 Feb 23.
In this study, we investigated the protective effect of selenium (Se) on cisplatin (Cis) induced testicular damage using histopathological, immunohistochemical and biochemical approaches. Twenty-one male Wistar rats were equally divided into three groups of seven rats each: control (C), Cis, and Cis+Se. Cis and Cis+Se group rats received Cis at a dose of 12mg/kg b.w./day, intraperitoneally for 3 consecutive days. Cis+Se group rats received selenium via oral gavage 3mg/kg/day (twice-a day as 1.5mg/kg) until 11th consecutive days starting at 5 days before cisplatin injection. C group received only 0.9% NaCl intraperitoneally and orally at same time and at equal volume. After the treatment, the histopathological, immunohistochemical and biochemical examinations were performed. In seminiferous tubules of Cis treated rats were observed the most consistent findings characterized with vacuolization, desquamation, disorganization, and also was a considerable reduction in elongated spermatids, however the Cis+Se group exhibited improved histopathologic changes. In the immunohistochemical examinations, caspase-3 immunopositive cells displayed higher in the Cis group according to C and Cis+Se groups. Bcl-2 and NF-κB staining revealed a moderate number in the C group and significantly fewer in the Cis group compared to the Cis+Se groups. Additionally, MDA levels were also significantly increased in the Cis group in comparison to Control group, but pretreatment with selenium prevented elevation of MDA levels significantly in Cis+Se group rats. This study indicates that Cis-treatment induced testicular apoptosis and lipid peroxidation, and combined treatment with selenium prevented severity of the toxicity in rats.
在本研究中，我们采用组织病理学、免疫组织化学和生物化学方法，研究了硒（Se）对顺铂（Cis）诱导的睾丸损伤的保护作用。将21只雄性Wistar大鼠平均分为三组，每组7只：对照组（C）、顺铂组（Cis）和顺铂+硒组（Cis+Se）。顺铂组和顺铂+硒组大鼠连续3天腹腔注射顺铂，剂量为12mg/kg体重/天。顺铂+硒组大鼠从顺铂注射前5天开始，通过口服灌胃给予硒，剂量为3mg/kg/天（分两次，每次1.5mg/kg），持续至第11天。对照组大鼠同时腹腔注射和口服等量的0.9%氯化钠。治疗后，进行组织病理学、免疫组织化学和生物化学检查。在顺铂处理的大鼠生精小管中观察到最一致的变化，其特征为空泡化、脱屑、结构紊乱，并且长形精子细胞数量显著减少，然而顺铂+硒组的组织病理学变化有所改善。在免疫组织化学检查中，与对照组和顺铂+硒组相比，顺铂组中caspase-3免疫阳性细胞较多。Bcl-2和NF-κB染色显示，对照组数量适中，与顺铂+硒组相比，顺铂组显著减少。此外，与对照组相比，顺铂组的丙二醛（MDA）水平也显著升高，但硒预处理可显著防止顺铂+硒组大鼠MDA水平升高。本研究表明，顺铂治疗可诱导睾丸细胞凋亡和脂质过氧化，而硒联合治疗可减轻大鼠的毒性严重程度。
