表没食子儿茶素没食子酸酯可拮抗顺铂对大鼠睾丸的毒性。
Epigallocatechin-3-gallate counters cisplatin toxicity of rat testes.
机构信息
a Biomedical Sciences Department, Division of Pharmacology, College of Medicine , King Faisal University , Al-Ahsa , Saudi Arabia.
b Department of Pharmacology, Faculty of Medicine , Minia University , El-Minia , Egypt.
出版信息
Pharm Biol. 2017 Dec;55(1):1710-1714. doi: 10.1080/13880209.2017.1322618.
CONTEXT
Epigallocatechin-3-gallate (EG), the main active flavonoid in green tea, has well-known anti-inflammatory, antioxidant, and anti-apoptotic activities.
OBJECTIVE
The EG protection against testicular injury induced by cisplatin was studied in Sprague-Dawley rats.
MATERIALS AND METHODS
Cisplatin (10 mg/kg, i.p) was given as a single injection to rats. EG was given at 40 and 80 mg/kg/day, i.p., for 5 days, starting the same day of cisplatin insult. Serum testosterone, and testicular malondialdehyde, total antioxidant status, nitric oxide, interleukin-6, interleukin-1β, cytochrome C, Bax/Bcl-2 ratio, and caspase-3 were measured. In addition, testicular histopathological examination and immunohistochemical expression of testicular tumour necrosis factor-α were evaluated.
RESULTS
Cisplatin, compared to the control, significantly decreased serum testosterone (6.48 ± 0.7 vs. 50.8 ± 4.91 ng/10 mL), and testicular tissue antioxidant status (17.3 ± 1.21 vs. 64.12 ± 5.4 μmol/g), and significantly increased interleukin-6 (85.81 ± 6.11 vs. 38.2 ± 2.79 pg/100 mg), interleukin-1β (98.09 ± 8.31 vs. 32.52 ± 2.08 pg/100 mg), malondialdehyde (74.5 ± 5.88 vs. 23.8 ± 1.91 nmol/g), nitric oxide (104.98 ± 8.5 vs. 52.68 ± 5.12 nmol/100 mg), cytochrome C (5.97 ± 0.33 vs. 1.6 ± 0.99 ng/mg protein), Bax/Bcl-2 ratio (4.01 ± 0.38 vs. 0.71 ± 0.0), and caspase-3 (3.2 ± 0.21 vs. 0.98 ± 0.08 O.D. 405 nm) in rat testes. EG (40 and 80 mg/kg, respectively) caused significant increases of serum testosterone (33.9 ± 2.89 and 47.88 ± 4.4 ng/10 mL), and testicular antioxidant status (47.1 ± 3.92 and 58.22 ± 3.58 μmol/g), and significant decreases of interleukin-6 (57.39 ± 4.2 and 48.18 ± 3.98 pg/100 mg), interleukin-1β (65.12 ± 5.88 and 41.96 ± 3.51 pg/100 mg), malondialdehyde (42.3 ± 3.9 and 28.67 ± 2.49 nmol/g), nitric oxide (70.6 ± 6.79 and 61.31 ± 5.18 nmol/100 mg), cytochrome C (3.4 ± 0.27 and 2.21 ± 0.18 ng/mg protein), Bax/Bcl-2 ratio (1.49 ± 0.14 and 1.1 ± 0.09), and caspase-3 (2.1 ± 0.17 and 1.48 ± 0.13 O.D. 405 nm) in testes of cisplatin-treated rats. Additionally, both doses of EG significantly ameliorated the histopathological injury and reduced tumour necrosis factor-α expression in rat testes.
CONCLUSION
EG can afford testicular protection in cisplatin-challenged rats by its antioxidant, antinitrative, anti-inflammatory and antiapoptotic effects.
背景
表没食子儿茶素没食子酸酯（EG）是绿茶中的主要活性类黄酮，具有众所周知的抗炎、抗氧化和抗凋亡作用。
目的
研究 EG 对顺铂诱导的大鼠睾丸损伤的保护作用。
材料和方法
顺铂（10mg/kg，腹腔注射）单次注射给予大鼠。EG 以 40 和 80mg/kg/天的剂量腹腔注射 5 天，从顺铂损伤的同一天开始。测量血清睾酮和睾丸丙二醛、总抗氧化状态、一氧化氮、白细胞介素-6、白细胞介素-1β、细胞色素 C、Bax/Bcl-2 比值和半胱天冬酶-3。此外，评估了睾丸组织病理学检查和肿瘤坏死因子-α的免疫组织化学表达。
结果
与对照组相比，顺铂显著降低了血清睾酮（6.48±0.7 与 50.8±4.91ng/10mL）和睾丸组织抗氧化状态（17.3±1.21 与 64.12±5.4μmol/g），显著增加了白细胞介素-6（85.81±6.11 与 38.2±2.79pg/100mg）、白细胞介素-1β（98.09±8.31 与 32.52±2.08pg/100mg）、丙二醛（74.5±5.88 与 23.8±1.91nmol/g）、一氧化氮（104.98±8.5 与 52.68±5.12nmol/100mg）、细胞色素 C（5.97±0.33 与 1.6±0.99ng/mg 蛋白）、Bax/Bcl-2 比值（4.01±0.38 与 0.71±0.0）和半胱天冬酶-3（3.2±0.21 与 0.98±0.08O.D.405nm）在大鼠睾丸中的表达。EG（40 和 80mg/kg）分别导致血清睾酮（33.9±2.89 和 47.88±4.4ng/10mL）和睾丸抗氧化状态（47.1±3.92 和 58.22±3.58μmol/g）显著增加，白细胞介素-6（57.39±4.2 和 48.18±3.98pg/100mg）、白细胞介素-1β（65.12±5.88 和 41.96±3.51pg/100mg）、丙二醛（42.3±3.9 和 28.67±2.49nmol/g）、一氧化氮（70.6±6.79 和 61.31±5.18nmol/100mg）、细胞色素 C（3.4±0.27 和 2.21±0.18ng/mg 蛋白）、Bax/Bcl-2 比值（1.49±0.14 和 1.1±0.09）和半胱天冬酶-3（2.1±0.17 和 1.48±0.13O.D.405nm）在顺铂处理的大鼠睾丸中显著降低。此外，EG 的两种剂量均显著改善了大鼠睾丸的组织病理学损伤，并降低了肿瘤坏死因子-α的表达。
结论
EG 通过其抗氧化、抗硝化、抗炎和抗凋亡作用，可为顺铂挑战的大鼠提供睾丸保护。
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