Joshi H, Subbarao S K, Raghavendra K, Sharma V P
Malaria Research Centre, Delhi, India.
Trans R Soc Trop Med Hyg. 1989 Mar-Apr;83(2):179-81. doi: 10.1016/0035-9203(89)90634-2.
185 isolates of Plasmodium vivax were collected from patients visiting the malaria clinic run by the National Malaria Eradication Programme, Delhi, India. Percoll gradient centrifugation was used to concentrate P. vivax parasites from 0.4 to 0.5 ml of blood collected by finger prick. The parasite concentrate from each isolate was electrophoretically analysed for lactate dehydrogenase (LDH), NADP-dependent glutamate dehydrogenase (GDH), glucose phosphate isomerase (GPI) and adenosine deaminase (ADA). Variations were observed in GPI, GDH and ADA systems. Four electrophoretic forms of GPI and 5 each of GDH and ADA were observed. Electrophoretic mobilities of the different isoenzymic forms in P. vivax were identical to those reported for P. falciparum, indicating that the 2 species cannot be differentiated on the basis of electrophoretic patterns of the 4 enzyme systems studied.
从印度德里国家疟疾根除计划疟疾诊所就诊的患者中收集了185株间日疟原虫。采用Percoll梯度离心法从手指针刺采集的0.4至0.5毫升血液中浓缩间日疟原虫寄生虫。对每个分离株的寄生虫浓缩物进行乳酸脱氢酶（LDH）、NADP依赖性谷氨酸脱氢酶（GDH）、葡萄糖磷酸异构酶（GPI）和腺苷脱氨酶（ADA）的电泳分析。在GPI、GDH和ADA系统中观察到了变异。观察到GPI有四种电泳形式，GDH和ADA各有五种电泳形式。间日疟原虫中不同同工酶形式的电泳迁移率与恶性疟原虫报道的相同，这表明基于所研究的4种酶系统的电泳图谱无法区分这两个物种。
