Zhang Weixing, Zhu Ming, Zhang Ge, Liu Feng, Wang Hongfang, Guo Xingqi, Xu Baohua
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
Naturwissenschaften. 2016 Apr;103(3-4):24. doi: 10.1007/s00114-016-1340-9. Epub 2016 Feb 27.
Estrogen-related receptor (ERR), which belongs to the nuclear receptor superfamily, has been implicated in diverse physiological processes involving the estrogen signaling pathway. However, little information is available on ERR in Apis cerana cerana. In this report, we isolated the ERR gene and investigated its involvement in antioxidant defense. Quantitative real-time polymerase chain reaction (qPCR) revealed that the highest mRNA expression occurred in eggs during different developmental stages. The expression levels of AccERR were highest in the muscle, followed by the rectum. The predicted transcription factor binding sites in the promoter of AccERR suggested that AccERR potentially functions in early development and in environmental stress responses. The expression of AccERR was induced by cold (4 °C), heat (42 °C), ultraviolet light (UV), HgCl2, and various types of pesticides (phoxim, deltamethrin, triadimefon, and cyhalothrin). Western blot was used to measure the expression levels of AccERR protein. These data suggested that AccERR might play a vital role in abiotic stress responses.
雌激素相关受体（ERR）属于核受体超家族，已被证明参与涉及雌激素信号通路的多种生理过程。然而，关于中华蜜蜂中ERR的信息却很少。在本报告中，我们分离了ERR基因并研究了其在抗氧化防御中的作用。定量实时聚合酶链反应（qPCR）显示，在不同发育阶段的卵中，mRNA表达量最高。中华蜜蜂ERR（AccERR）的表达水平在肌肉中最高，其次是直肠。AccERR启动子中预测的转录因子结合位点表明，AccERR可能在早期发育和环境应激反应中发挥作用。AccERR的表达受到寒冷（4℃）、高温（42℃）、紫外线（UV）、HgCl2以及各种农药（辛硫磷、溴氰菊酯、三唑酮和氯氰菊酯）的诱导。采用蛋白质免疫印迹法检测AccERR蛋白的表达水平。这些数据表明，AccERR可能在非生物胁迫反应中发挥重要作用。
