Gherlone Enrico Felice, Capparé Paolo, Pasciuta Renée, Grusovin Maria Gabriella, Mancini Nicasio, Burioni Roberto
Dental School, Vita-Salute University, Milan, Italy and Department of Dentistry, IRCCS San Raffaele Hospital, Milan, Italy.
Laboratory of Microbiology and Virology, Vita-Salute San Raffaele University, Milan, Italy.
New Microbiol. 2016 Jan;39(1):49-56.
The aim of the present in vitro study was to evaluate bacterial microleakage from inside to outside the implant-abutment assembly in a new design of internal conical connection compared to eight different internal connections. The design of this connection should prevent or limit microbiologic leakage into the surrounding implant tissue, that could contribute to infections without bone loss (mucositis) or with bone loss (peri-implantits). In order to investigate bacterial microleakage, the inner part of each system was inoculated with an Escherichia coli suspension. Eight different groups were considered; each group was composed of 10 dental implants, for a total of 80 implants. Groups 1-7 were considered controls, while group 8 was the test connection (an internal connection characterized by a double taper principle). Results showed that in control implants (Group 1 to 7), little microleakage was observed after the first 6 hours (500 CFU/ μl) and, after 24 hours of incubation, they showed a significant bacterial contamination in all samples (>100.000 CFU/ μl). In group 8 (test connection) no contamination was found in the first 6 hours, with 7 out of 10 implants showing no contamination even after 96 hours. Statistically significant differences were found between Group 8 and the other groups (p<0.05), whereas no significant differences were found among implants of the control groups (from group 1 to 7). Within the limits of the present study, the new connection studied presented significantly less microleakage at 96 h in comparison with the other control internal connections.
本体外研究的目的是评估一种新型内锥连接设计的种植体-基台组件从内部到外部的细菌微渗漏情况，并与八种不同的内部连接进行比较。这种连接设计应能防止或限制微生物渗漏到周围的种植体组织中，因为这可能导致无骨质流失的感染（黏膜炎）或有骨质流失的感染（种植体周围炎）。为了研究细菌微渗漏情况，在每个系统的内部接种了大肠杆菌悬液。研究了八个不同的组；每组由10颗牙种植体组成，总共80颗种植体。第1-7组为对照组，而第8组为测试连接组（一种采用双锥原理的内部连接）。结果显示，在对照种植体（第1至7组）中，最初6小时后观察到少量微渗漏（500 CFU/μl），孵育24小时后，所有样本均显示出显著的细菌污染（>100,000 CFU/μl）。在第8组（测试连接组）中，最初6小时未发现污染，10颗种植体中有7颗即使在96小时后也未发现污染。第8组与其他组之间存在统计学显著差异（p<0.05），而对照组（第1至7组）的种植体之间未发现显著差异。在本研究的范围内，与其他对照内部连接相比，所研究的新型连接在96小时时的微渗漏明显更少。
