Grando S A
Vestn Dermatol Venerol. 1989(9):10-3.
The epidermal keratinocyte (EK) culture supernatant from patients with pemphigus vulgaris and bullous pemphigoid in the acute phase of the disease depressed the lymphocyte blastogenesis with phytohemagglutinin and conA, interleukin-1 (IL-1) and interleukin-2 (IL-2) production, and the expression of receptors to IL-2 by donor peripheral blood mononuclears. During remission EK recovered their ability to stimulate the immunocompetent cell functional activity. Addition of pemphigus and pemphigoid antibodies to normal human EK culture resulted in the emergence of immune reaction suppressing factors in the supernatant. Reduction of the lymphocyte functional activity, induced by patients' antibody-transformed EK, normalized after blood adsorption on carbon hemocarbosorbents. IL-1 and IL-2 inhibitors have been detected in eluates from sorbents.
寻常型天疱疮和大疱性类天疱疮急性期患者的表皮角质形成细胞（EK）培养上清液可抑制供体外周血单个核细胞通过植物血凝素和刀豆蛋白A进行的淋巴细胞增殖、白细胞介素-1（IL-1）和白细胞介素-2（IL-2）的产生以及IL-2受体的表达。在缓解期，EK恢复了刺激免疫活性细胞功能活性的能力。向正常人EK培养物中添加天疱疮和类天疱疮抗体导致上清液中出现免疫反应抑制因子。患者抗体转化的EK诱导的淋巴细胞功能活性降低在血液吸附于碳血液吸附剂后恢复正常。在吸附剂的洗脱液中检测到了IL-1和IL-2抑制剂。
