Grando S A, Glukhenky B T, Drannik G N, Kostromin A P, Chernyavsky A I, Barabash T M
Department of Dermatology, University of Minnesota, Minneapolis 55455-0392.
J Clin Lab Immunol. 1990 Jul;32(3):137-41.
The aim of this study was to reveal the origin of interleukin cascade reaction disturbances in pemphigus vulgaris (PV) and bullous pemphigoid (BP). We investigated the influence of culture supernatant of epidermal keratinocytes from the skin of PV and BP patients on functional activity of donor peripheral blood mononuclears. It was found that epidermal keratinocyte culture supernatant in the acute phase of PV and BP decreased the ability of mononuclears to proliferate in response to PHA and Con A, secrete interleukin-1 and interleukin-2, and absorb an exogenous interleukin-2. Such inhibitory effect was far less pronounced in epidermal keratinocytes obtained from PV and BP patients at the remission stage. Epidermocytes from healthy subjects stimulated the immunocompetent cell activity. The results obtained indicate that epidermal keratinocytes in the acute phase of pemphigus and pemphigoid produce a putative soluble factor(s), which may be the origin of the immunodeficiency in PV and BP patients.
本研究的目的是揭示寻常型天疱疮（PV）和大疱性类天疱疮（BP）中白细胞介素级联反应紊乱的起源。我们研究了PV和BP患者皮肤的表皮角质形成细胞培养上清液对供体外周血单个核细胞功能活性的影响。结果发现，PV和BP急性期的表皮角质形成细胞培养上清液降低了单个核细胞对PHA和Con A刺激的增殖能力、白细胞介素-1和白细胞介素-2的分泌能力以及对外源白细胞介素-2的吸收能力。在缓解期从PV和BP患者获取的表皮角质形成细胞中，这种抑制作用要弱得多。健康受试者的表皮细胞可刺激免疫活性细胞的活性。所得结果表明，天疱疮和类天疱疮急性期的表皮角质形成细胞产生一种假定的可溶性因子，这可能是PV和BP患者免疫缺陷的根源。
