聚精氨酸共轭三芳甲基自由基作为细胞内自旋标记物。
Poly-arginine conjugated triarylmethyl radical as intracellular spin label.
作者信息
Driesschaert Benoit, Bobko Andrey A, Eubank Timothy D, Samouilov Alexandre, Khramtsov Valery V, Zweier Jay L
机构信息
In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV 26506, United States; Department of Biochemistry, West Virginia University School of Medicine, Morgantown, WV 26506, United States.
In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV 26506, United States; Department of Microbiology, Immunology & Cell Biology, West Virginia University School of Medicine, Morgantown, WV 26506, United States.
出版信息
Bioorg Med Chem Lett. 2016 Apr 1;26(7):1742-4. doi: 10.1016/j.bmcl.2016.02.048. Epub 2016 Feb 19.
Abstract
Stable triarylmethyl radicals are ideal spin labels used for biomedical electron paramagnetic resonance applications. Previously reported structures exhibit polar charged functions for water solubilization preventing them from crossing the cell membrane. We report the synthesis of a triarylmethyl radical conjugated to poly-arginine peptide allowing intracellular delivery of the paramagnetic label.
摘要
稳定的三芳基甲基自由基是用于生物医学电子顺磁共振应用的理想自旋标记物。先前报道的结构具有用于增溶的极性带电官能团，这使得它们无法穿过细胞膜。我们报道了一种与聚精氨酸肽共轭的三芳基甲基自由基的合成，该合成使得顺磁标记物能够进行细胞内递送。
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