一种分离高度富集的肠道干细胞群体的通用策略。
A Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells.
作者信息
Nefzger Christian M, Jardé Thierry, Rossello Fernando J, Horvay Katja, Knaupp Anja S, Powell David R, Chen Joseph, Abud Helen E, Polo Jose M
机构信息
Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, VIC 3800, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, VIC 3800, Australia; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC 3800, Australia.
Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, VIC 3800, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, VIC 3800, Australia; Cancer Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, VIC 3800, Australia; Centre for Cancer Research, Hudson Institute of Medical Research, 27-31 Wright Street, Clayton, VIC 3168, Australia.
出版信息
Stem Cell Reports. 2016 Mar 8;6(3):321-9. doi: 10.1016/j.stemcr.2016.01.014. Epub 2016 Feb 25.
The isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population transcriptionally and functionally equivalent to the gold standard Lgr5-GFP ISCs. Used on reporter-free mice, this strategy allows the isolation of functional, transcriptionally distinct ISCs uncompromised by Lgr5 haploinsufficiency.
分离纯净的小鼠肠道干细胞（ISC）群体对于促进组织稳态、组织再生和肠道疾病的功能研究至关重要。然而，ISC的纯化主要依赖于在小鼠中使用转基因报告等位基因。在这里，我们介绍一种组合细胞表面标志物介导的策略，该策略能够分离出在转录和功能上与金标准Lgr5-GFP ISC等效的ISC群体。将该策略应用于无报告基因的小鼠，能够分离出不受Lgr5单倍剂量不足影响的功能性、转录上不同的ISC。
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