来自中国的竹子花叶病毒重组分离株的分子特征及检测

Molecular characterization and detection of a recombinant isolate of bamboo mosaic virus from China.

作者信息

Lin Wenwu, Gao Fangluan, Yang Wenting, Yu Chaowei, Zhang Jie, Chen Lingli, Wu Zujian, Hsu Yau-Heiu, Xie Lianhui

机构信息

Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian.

Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, 40227, Taiwan.

出版信息

Arch Virol. 2016 Apr;161(4):1091-4. doi: 10.1007/s00705-016-2773-3. Epub 2016 Feb 29.

DOI:10.1007/s00705-016-2773-3

PMID:26923929

Abstract

The complete genome sequences of three isolates of bamboo mosaic virus (BaMV) from mainland China were determined and compared to those of BaMV isolates from Taiwan. Sequence analysis showed that isolate BaMV-JXYBZ1 from Fuzhou shares 98 % nucleotide sequence identity with BaMV-YTHSL14 from nucleotides 2586 to 6306, and more than 94 % nucleotide sequence identity with BaMV-MUZHUBZ2 in other regions. Recombination and phylogenetic analyses indicate that BaMV-JXYBZ1 is a recombinant with one recombination breakpoint. To our knowledge, this is the first report of a BaMV recombinant worldwide.

摘要

测定了来自中国大陆的三株竹花叶病毒（BaMV）分离株的全基因组序列，并与来自台湾的BaMV分离株进行了比较。序列分析表明，来自福州的分离株BaMV-JXYBZ1在核苷酸2586至6306处与BaMV-YTHSL14的核苷酸序列同一性为98%，在其他区域与BaMV-MUZHUBZ2的核苷酸序列同一性超过94%。重组和系统发育分析表明，BaMV-JXYBZ1是一个具有一个重组断点的重组体。据我们所知，这是全球首次报道BaMV重组体。