Lin N S, Hsu Y H
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China.
Virology. 1994 Aug 1;202(2):707-14. doi: 10.1006/viro.1994.1392.
A small RNA molecule with properties of a satellite RNA was found in an isolate of bamboo mosaic potexvirus (BaMV-V) from Bambusa vulgaris McClure. This RNA (sBaMV RNA) and the genomic RNA of BaMV shared no significant sequence homology as assessed by hybridization with cDNA probes derived from the genomes of BaMV and sBaMV RNA. Replication of sBaMV RNA in barley protoplasts or Chenopodium quinoa was supported by BaMV, but not by potato virus X, the type member of the potexvirus group, or other unrelated viruses. The complete nucleotide sequence of sBaMV RNA is 836 nucleotides (excluding the poly(A) tail) and contains an open reading frame which starts after a 159-nucleotide 5'-untranslated region and encodes a 20,154-mol wt protein. In an in vitro rabbit reticulocyte lysate system, sBaMV RNA directed the synthesis of a protein estimated to be 25 kDa by SDS-polyacrylamide gel electrophoresis. The sBaMV RNA-encoded protein was not immunoprecipitated with antiserum against BaMV capsid protein. However, the sBaMV RNA was encapsidated with BaMV capsid protein to form rod-shaped particles with an average length of 60 nm as shown by immunoelectron microscopy. This is the first satellite RNA found in the potexvirus group.
在一种来自簕竹（Bambusa vulgaris McClure）的竹花叶病毒分离株（BaMV-V）中发现了一种具有卫星RNA特性的小RNA分子。通过与源自BaMV和sBaMV RNA基因组的cDNA探针杂交评估，这种RNA（sBaMV RNA）与BaMV的基因组RNA没有显著的序列同源性。sBaMV RNA在大麦原生质体或藜麦（Chenopodium quinoa）中的复制由BaMV支持，但不由马铃薯X病毒（Potexvirus属的模式成员）或其他无关病毒支持。sBaMV RNA的完整核苷酸序列为836个核苷酸（不包括poly(A)尾），并包含一个开放阅读框，该开放阅读框在159个核苷酸的5'非翻译区之后开始，编码一个20,154道尔顿分子量的蛋白质。在体外兔网织红细胞裂解物系统中，sBaMV RNA通过SDS-聚丙烯酰胺凝胶电泳指导合成一种估计为25 kDa的蛋白质。sBaMV RNA编码的蛋白质不能用抗BaMV衣壳蛋白的抗血清进行免疫沉淀。然而，如免疫电子显微镜所示，sBaMV RNA被BaMV衣壳蛋白包裹形成平均长度为60 nm的杆状颗粒。这是在Potexvirus属中发现的第一个卫星RNA。
