Tomino Y, Funabiki K, Shirato I, Koide H, Eguchi K, Yagame M, Sakai H
Department of Medicine, Juntendo University, School of Medicine, Tokyo, Japan.
Acta Pathol Jpn. 1989 May;39(5):296-305. doi: 10.1111/j.1440-1827.1989.tb02439.x.
Computer imaging analysis was used for quantitative evaluation of the extents, amounts and distributions of glomerular extracellular components, such as the 7S and NC-1 domains of type IV collagen, laminin (LN), fibronectin (FN) and IgA, in glomeruli from patients with IgA nephropathy. Renal biopsy specimens from 13 patients with IgA nephropathy were incubated with mouse monoclonal antibodies against the FN or non-collagenous (NC-1) domain of type IV collagen or polyclonal antiserum against the LN or 7S domain of human type IV collagen, and then stained with appropriate dilutions of FITC-labeled anti-mouse Ig antisera. Marked staining of the 7S or NC-1 domain of type IV collagen, LN or FN was detected in the glomerular capillary walls and/or mesangial areas in patients with IgA nephropathy. In particular, a prominent increase of FN was observed in the subendothelial regions of glomerular capillary walls, i.e. mesangial interposition, in the moderate or advanced stage of IgA nephropathy. Therefore, computer imaging analysis was shown to be useful for the quantitative determination of such components distributed in glomeruli from patients with IgA nephropathy.
计算机成像分析用于定量评估IgA肾病患者肾小球细胞外成分的范围、数量和分布，如IV型胶原的7S和NC-1结构域、层粘连蛋白（LN）、纤连蛋白（FN）和IgA。13例IgA肾病患者的肾活检标本与抗FN或IV型胶原非胶原（NC-1）结构域的小鼠单克隆抗体或抗人IV型胶原LN或7S结构域的多克隆抗血清孵育，然后用适当稀释的异硫氰酸荧光素（FITC）标记的抗小鼠Ig抗血清染色。在IgA肾病患者的肾小球毛细血管壁和/或系膜区检测到IV型胶原的7S或NC-1结构域、LN或FN的明显染色。特别是，在IgA肾病的中度或晚期，在肾小球毛细血管壁的内皮下区域，即系膜插入处，观察到FN显著增加。因此，计算机成像分析被证明可用于定量测定IgA肾病患者肾小球中分布的此类成分。
