Tomino Y, Sakai H, Miura M, Suga T, Yagame M, Endoh M, Nomoto Y
Am J Nephrol. 1986;6(3):187-92. doi: 10.1159/000167112.
A study of double immunofluorescence-staining of immunoglobulins and sialic acids in the glomeruli from patients with IgA nephropathy is described. Renal biopsy specimens from patients with IgA nephropathy were stained with rhodamine-labeled antihuman IgA, IgG or IgM antisera and then stained with FITC-labeled Limulus polyphemus (LPA), Tricum vulgaris (WGA) or antihuman C3 antisera. Marked positive stainings of IgA and C3 and positive binding of LPA or WGA were observed in the glomerular mesangial areas from patients with IgA nephropathy. LPA or WGA were not bound with glomerular capillary walls from patients with moderate and advanced stages of IgA nephropathy, although depositions of IgA and C3 were markedly observed in such walls. There was a significant inverse correlation between the deposition of IgA and the binding of LPA or WGA in glomerular capillary walls obtained from these patients with IgA nephropathy. The levels of proteinuria from patients with moderate and advanced stages of IgA nephropathy were significantly higher than those with minimal and slight stages of such disease. It is suggested that the decrease of sialic acids in glomerular capillary walls might be due to a deposition of IgA in some patients with IgA nephropathy. It is concluded that high levels of proteinuria might be due to the decrease of sialic acids in glomerular capillary walls from patients with moderate and advanced stages of IgA nephropathy.
本文描述了一项对IgA肾病患者肾小球中免疫球蛋白和唾液酸进行双重免疫荧光染色的研究。用罗丹明标记的抗人IgA、IgG或IgM抗血清对IgA肾病患者的肾活检标本进行染色，然后用异硫氰酸荧光素标记的鲎试剂（LPA）、小麦胚凝集素（WGA）或抗人C3抗血清进行染色。在IgA肾病患者的肾小球系膜区观察到IgA和C3的明显阳性染色以及LPA或WGA的阳性结合。尽管在IgA肾病中晚期患者的肾小球毛细血管壁中明显观察到IgA和C3的沉积，但LPA或WGA并未与这些患者的肾小球毛细血管壁结合。在这些IgA肾病患者的肾小球毛细血管壁中，IgA的沉积与LPA或WGA的结合之间存在显著的负相关。IgA肾病中晚期患者的蛋白尿水平明显高于该病轻度和极轻度阶段的患者。提示在一些IgA肾病患者中，肾小球毛细血管壁中唾液酸的减少可能是由于IgA的沉积所致。得出的结论是，蛋白尿水平升高可能是由于IgA肾病中晚期患者肾小球毛细血管壁中唾液酸减少所致。
