Hardy M D, Konetski D, Bowman C N, Devaraj N K
Department of Chemistry and Biochemistry, University of California, San Diego, CA 92093, USA.
Department of Chemical and Biological Engineering, University of Colorado, Boulder, Boulder, CO 80309, USA.
Org Biomol Chem. 2016 Jun 15;14(24):5555-8. doi: 10.1039/c6ob00290k.
As more methodologies for generating and manipulating biomimetic cellular systems are developed, opportunities arise for combining different methods to create more complex synthetic biological constructs. This necessitates an increasing need for tools to selectively trigger individual methodologies. Here we demonstrate ruthenium tris-bipyridine mediated photoredox triggering of the copper catalyzed alkyne azide cycloaddition reaction (CuAAC), resulting in the synthesis of biomimetic phospholipids in situ, and subsequent membrane assembly. The use of a ruthenium-copper electron transport chain to trigger phospholipid assembly opens up future opportunities for spatiotemporal synthesis of membranes.
随着越来越多用于生成和操纵仿生细胞系统的方法被开发出来，将不同方法结合以创建更复杂的合成生物构建体的机会也随之出现。这就越来越需要有工具来选择性地触发各个方法。在此，我们展示了三联吡啶钌介导的光氧化还原触发铜催化的炔烃叠氮环加成反应（CuAAC），从而原位合成仿生磷脂并随后进行膜组装。利用钌 - 铜电子传输链来触发磷脂组装为膜的时空合成开辟了未来的机会。
