a Department of Chemistry , University of Sistan and Baluchestan , P.O. Box 98155-147, Zahedan , Iran.
J Biomol Struct Dyn. 2017 Feb;35(2):300-311. doi: 10.1080/07391102.2015.1137491. Epub 2016 Apr 14.
The binding of [Dy(dmp)Cl(OH)], where dmp is 2,9-dimethyl 1,10-phenanthroline, with Fish salmon DNA (FS-DNA) is investigated by absorption and emission spectroscopy, quenching studies, salt dependent, and gel electrophoresis. The binding constant (K) of the interaction is calculated as (1.27 ± .05) × 10 M from absorption spectral titration data. The Stern-Volmer constant (K), thermodynamic parameters involves ΔG°, ∆H°, and ∆S° are calculated by fluorescent data and Van't Hoff equation. The thermodynamic studies show that the reaction for the binding of the complex with FS-DNA is endothermic and entropically driven (ΔS° > 0, ΔH° > 0). The effect of the complex concentration on FS-DNA cleavage reactions is also investigated by gel electrophoresis. Furthermore, the Dy(III) complex has been screened for its antibacterial activity. The experimental results suggest that the Dy(III) complex binds significantly to FS-DNA by hydrophobic groove binding mode and the complex has more efficient antibacterial activity compared to its metal salt.
用吸收和荧光光谱法、荧光猝灭研究、盐依赖性和凝胶电泳法研究了[Dy(dmp)Cl(OH)]（其中 dmp 是 2,9-二甲基 1,10-菲啰啉）与鲑鱼 DNA（FS-DNA）的结合。从吸收光谱滴定数据计算出相互作用的结合常数（K）为（1.27 ± 0.05）×10 M。通过荧光数据和范特霍夫方程计算出 Stern-Volmer 常数（K）、热力学参数涉及 ΔG°、∆H° 和 ∆S°。热力学研究表明，配合物与 FS-DNA 的结合反应是吸热的，并且是熵驱动的（ΔS°>0，ΔH°>0）。还通过凝胶电泳研究了配合物浓度对 FS-DNA 断裂反应的影响。此外，还对 Dy(III)配合物的抗菌活性进行了筛选。实验结果表明，Dy(III)配合物通过疏水沟结合模式与 FS-DNA 显著结合，并且与金属盐相比，该配合物具有更高的抗菌活性。
