Sakai Kazuaki, Loza Ernesto, Roig Guido Villa-Gomez, Nozaki Ryoko, Asai Takao, Ikoma Toshikazu, Tsuchiya Yasuo, Kiyohara Chikako, Yamamoto Masaharu, Nakamura Kazutoshi
Division of Preventive Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan E-mail :
Asian Pac J Cancer Prev. 2016;17(2):781-4. doi: 10.7314/apjcp.2016.17.2.781.
The Plurinational State of Bolivia (Bolivia) has a high incidence rate of gallbladder cancer (GBC). However, the genetic and environmental risk factors for GBC development are not well understood. We aimed to assess whether or not cytochrome P450 (CYP1A1), glutathione S-transferase mu 1 (GSTM1), theta 1 (GSTT1) and tumor suppressor protein p53 (TP53) genetic polymorphisms modulate GBC susceptibility in Bolivians. This case-control study covered 32 patients with GBC and 86 healthy subjects. GBC was diagnosed on the basis of histological analysis of tissues at the Instituto de Gastroenterologia Boliviano-Japones (IGBJ); the healthy subjects were members of the staff at the IGBJ. Distributions of the CYP1A1 rs1048943 and TP53 rs1042522 polymorphisms were assayed using PCR-restriction fragment length polymorphism assay. GSTM1 and GSTT1 deletion polymorphisms were detected by a multiplex PCR assay. The frequency of the GSTM1 null genotype was significantly higher in GBC patients than in the healthy subjects (odds ratio [OR], 2.35; 95% confidence interval [CI], 1.03-5.37; age-adjusted OR, 3.53; 95% CI, 1.29-9.66; age- and sex-adjusted OR, 3.40; 95% CI, 1.24-9.34). No significant differences were observed in the frequencies of CYP1A1, GSTT1, or TP53 polymorphisms between the two groups. The GSTM1 null genotype was associated with increased GBC risk in Bolivians. Additional studies with larger control and case populations are warranted to confirm the association between the GSTM1 deletion polymorphism and GBC risk suggested in the present study.
多民族玻利维亚国（玻利维亚）的胆囊癌（GBC）发病率很高。然而，GBC发生的遗传和环境风险因素尚未得到充分了解。我们旨在评估细胞色素P450（CYP1A1）、谷胱甘肽S-转移酶μ1（GSTM1）、θ1（GSTT1）和肿瘤抑制蛋白p53（TP53）基因多态性是否会调节玻利维亚人患GBC的易感性。这项病例对照研究涵盖了32例GBC患者和86名健康受试者。GBC是根据玻利维亚-日本胃肠病学研究所（IGBJ）组织的组织学分析诊断出来的；健康受试者是IGBJ的工作人员。使用聚合酶链反应-限制性片段长度多态性分析来检测CYP1A1 rs1048943和TP53 rs1042522多态性的分布。通过多重聚合酶链反应分析检测GSTM1和GSTT1缺失多态性。GBC患者中GSTM1无效基因型的频率显著高于健康受试者（优势比[OR]，2.35；95%置信区间[CI]，1.03 - 5.37；年龄调整后的OR，3.53；95%CI，1.29 - 9.66；年龄和性别调整后的OR，3.40；95%CI，1.24 - 9.34）。两组之间CYP1A1、GSTT1或TP53多态性的频率没有观察到显著差异。GSTM1无效基因型与玻利维亚人GBC风险增加有关。有必要进行更多有更大对照组和病例组的研究，以证实本研究中提示的GSTM1缺失多态性与GBC风险之间的关联。
