Malik Manzoor Ahmad, Malik Subzar Ahmad, Haq Malik Gowharul, Bangri Sadaf Ali, Ahmad Sheikh Zahoor, Shah Omar Javed, Shah Zafar Amin
Cancer Diagnostic & Research Centre, Department of Immunology & Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences (SKIMS), Soura, Srinagar, Kashmir, India.
Department of Immunology & Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences (SKIMS), Soura, Srinagar, Kashmir, India.
Nutr Cancer. 2020;72(6):984-991. doi: 10.1080/01635581.2019.1661503. Epub 2019 Nov 22.
Glutathionetransferase T1 (GSTT1) and glutathionetransferase M1 (GSTM1) genes are associated with increase susceptibility to developing different types of cancers. The aim of present study was to investigate the role of genetic variants of GSTM1 and GSTT1 in gallbladder cancer (GBC) and cholelithiasis in Kashmir valley. Genotyping was done by multiplex polymerase chain reaction in 100 GBC, 100 cholelithiasis, and 150 controls adjusted by age and sex. We also performed a meta-analysis of published studies on GSTM1 and GSTT1 to evaluate the association between the GSTM1 and GSTT1 polymorphisms and GBC. Pooled odds ratio (OR) and 95% confidence interval (CI) were calculated using random- or fixed-effects model. In the present study, no association was observed between GSTM1 null and GSTT1 null genotypes and GBC and cholelithiasis. Meta-analysis results showed that GSTM1 null genotype was associated with GBC risk ( 0.042). Subgroup analysis by ethnicity showed that GSTM1 null ( 0.024) and GSTT1 null genotype ( 0.037) were significantly associated with risk of GBC in Asians. This is the first study to investigate the role of genetic variants of GSTM1 and GSTT1 in GBC in Kashmir valley and cholelithiasis in the world.
谷胱甘肽S-转移酶T1（GSTT1）和谷胱甘肽S-转移酶M1（GSTM1）基因与患不同类型癌症的易感性增加有关。本研究的目的是调查GSTM1和GSTT1基因变异在克什米尔山谷胆囊癌（GBC）和胆石症中的作用。通过多重聚合酶链反应对100例GBC患者、100例胆石症患者和150例按年龄和性别匹配的对照进行基因分型。我们还对已发表的关于GSTM1和GSTT1的研究进行了荟萃分析，以评估GSTM1和GSTT1基因多态性与GBC之间的关联。使用随机或固定效应模型计算合并比值比（OR）和95%置信区间（CI）。在本研究中，未观察到GSTM1缺失和GSTT1缺失基因型与GBC和胆石症之间存在关联。荟萃分析结果显示，GSTM1缺失基因型与GBC风险相关（OR = 0.042）。按种族进行的亚组分析表明，GSTM1缺失（OR = 0.024）和GSTT1缺失基因型（OR = 0.037）与亚洲人患GBC的风险显著相关。这是第一项研究GSTM1和GSTT1基因变异在克什米尔山谷GBC和世界胆石症中作用的研究。
