Dartsch P C, Bauriedel G, Schinko I, Weiss H D, Höfling B, Betz E
Institute of Physiology I, University of Tübingen, F.R.G.
Atherosclerosis. 1989 Dec;80(2):149-57. doi: 10.1016/0021-9150(89)90023-3.
The Simpson atherectomy device used for the recanalization of severely stenosed peripheral arteries is able to collect plaque material which can be further characterized. This study reports histological, immunohistochemical and transmission electron microscopic findings on advanced human primary atherosclerotic plaques of peripheral arteries percutaneously removed by a Simpson atherectomy catheter. Material from stenosing plaques consisted of dense connective tissue with abundant amounts of concentrically arranged elastic fibers and lamellae. This meshwork contained numerous cells, often arranged in clusters and oriented with their longer axis parallel to the direction of blood flow. The vast majority of these cells could be easily identified as vimentin-positive and desmin-negative smooth muscle cells containing lipid deposits in the perinuclear region and numerous glycogen particles. Monocytes/macrophages were observed only very infrequently. Plaque tissue contained a range of smooth muscle cell phenotypes. Most of the cells were of an intermediate phenotype, i.e. sparsely filled with myofilament bundles at the cell periphery and a high amount of organelles such as mitochondria, rough endoplasmic reticulum and Golgi cisterns. An intact lining of pieces of intimal tissue with endothelial cells was not observed. Two-dimensional gel electrophoresis of plaque tissue showed the presence of alpha-, beta- and gamma-actin isoforms with a clear predominance of the beta-isoform.
用于严重狭窄外周动脉再通的Simpson旋切装置能够收集可进一步表征的斑块物质。本研究报告了通过Simpson旋切导管经皮切除的外周动脉晚期人类原发性动脉粥样硬化斑块的组织学、免疫组织化学和透射电子显微镜检查结果。狭窄斑块的物质由致密结缔组织组成，含有大量同心排列的弹性纤维和薄片。这个网络包含许多细胞，通常成簇排列，其长轴与血流方向平行。这些细胞中的绝大多数很容易被鉴定为波形蛋白阳性、结蛋白阴性的平滑肌细胞，在核周区域含有脂质沉积和大量糖原颗粒。单核细胞/巨噬细胞仅偶尔观察到。斑块组织包含一系列平滑肌细胞表型。大多数细胞为中间表型，即细胞周边稀疏地充满肌丝束，并且含有大量细胞器，如线粒体、粗面内质网和高尔基池。未观察到带有内皮细胞的内膜组织碎片的完整内衬。斑块组织的二维凝胶电泳显示存在α-、β-和γ-肌动蛋白同工型，其中β-同工型明显占优势。
