Bhardwaj Ruchika, Kumar Ritesh, Singh Sanjeev Kumar, Selvaraj Chandrabose, Dubey Vikash Kumar
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
Computer Aided Drug Designing and Molecular Modeling Laboratory, Department of Bioinformatics, Alagappa University, Karaikudi, Tamil Nadu 630 004, India.
Arch Biochem Biophys. 2016 Apr 15;596:10-21. doi: 10.1016/j.abb.2016.02.025. Epub 2016 Feb 27.
The genome of Leishmania donovani, the causative agent of visceral leishmaniasis, codes for approximately 65% of both conserved and non-conserved hypothetical proteins. Studies on 'conserved hypothetical' proteins are expected to reveal not only new and crucial aspects of Leishmania biochemistry, but it could also lead to discovery of novel drug candidates. Conserved hypothetical protein, LdBPK_070020, is a 31.14 kDa protein, encoded by an 810 bp gene. BLAST analysis of LdBPK_070020, performed against NCBI non-redundant database, showed 80-99% similarity with conserved hypothetical proteins of Leishmania belonging to other species. Using homologues recombination method, we have performed gene knockout of LdBPK_070020 and effects of the same were investigated on the parasite. The gene knocked out strain shows significant retardation in growth with respect to wild type. Detailed biochemical studies indicated towards important role of LdBPK_070020 in the parasite survival and growth.
内脏利什曼病的病原体杜氏利什曼原虫的基因组编码了约65%的保守和非保守假设蛋白。对“保守假设”蛋白的研究不仅有望揭示利什曼原虫生物化学的新的关键方面，还可能导致发现新的候选药物。保守假设蛋白LdBPK_070020是一种31.14 kDa的蛋白，由一个810 bp的基因编码。针对NCBI非冗余数据库对LdBPK_070020进行的BLAST分析显示，它与其他物种的利什曼原虫的保守假设蛋白有80-99%的相似性。我们使用同源重组方法对LdBPK_070020进行了基因敲除，并研究了其对寄生虫的影响。基因敲除菌株相对于野生型显示出明显的生长迟缓。详细的生化研究表明LdBPK_070020在寄生虫的存活和生长中起重要作用。
