杜氏利什曼原虫CAAX异戊二烯基蛋白酶II作为潜在药物靶点的评估：基因敲除后，该寄生虫的感染性和生长显著降低。
Evaluation of CAAX prenyl protease II of Leishmania donovani as potential drug target: Infectivity and growth of the parasite is significantly lowered after the gene knockout.
作者信息
Bhardwaj Ruchika, Das Mousumi, Singh Shalini, Chiranjivi Adarsh Kumar, Prabhu Sitraraau Vijaya, Singh Sanjeev Kumar, Dubey Vikash Kumar
机构信息
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
Computer Aided Drug Designing and Molecular Modeling Laboratory, Department of Bioinformatics, Alagappa University, Karaikudi, Tamil Nadu 630 004, India.
出版信息
Eur J Pharm Sci. 2017 May 1;102:156-160. doi: 10.1016/j.ejps.2017.03.005. Epub 2017 Mar 7.
Prenylation pathway is responsible for post translational modification of various signal proteins, including proteins of Ras superfamily. CAAX prenyl proteases are known to be key players in prenylation pathway. In the current study, we have evaluated CAAX prenyl protease II as a possible drug target against Leishmania donovani parasite, the causative agent of visceral leishmaniasis. Gene knockout strategy was employed to target CAAX prenyl protease II and subsequent effects were studied. CAAX prenyl protease II knockout resulted in significant decrease in growth and infectivity.
异戊二烯化途径负责各种信号蛋白的翻译后修饰，包括Ras超家族的蛋白。已知CAAX异戊二烯基蛋白酶是异戊二烯化途径中的关键参与者。在当前的研究中，我们评估了CAAX异戊二烯基蛋白酶II作为针对杜氏利什曼原虫（内脏利什曼病的病原体）的潜在药物靶点。采用基因敲除策略靶向CAAX异戊二烯基蛋白酶II，并研究其后续影响。CAAX异戊二烯基蛋白酶II基因敲除导致生长和感染性显著降低。
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