Sun Mou-Jong, Cheng Yu-Shan, Sun Ryan, Cheng Wen-Ling, Liu Chin-San
Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua, Taiwan; General Education Center, Chienkuo Technology University, Changhua, Taiwan; Department of Vascular and Genomics Center, Changhua Christian Hospital, Changhua, Taiwan.
Department of Vascular and Genomics Center, Changhua Christian Hospital, Changhua, Taiwan.
Taiwan J Obstet Gynecol. 2016 Feb;55(1):9-15. doi: 10.1016/j.tjog.2014.04.032.
The study aimed to explore the changes in mitochondrial DNA (mtDNA) copy number, collagen, and matrix metalloproteinase (MMPs) expression with pelvic organ prolapse (POP) in the uterosacral ligaments of premenopausal women.
A group of 56 premenopausal women, all younger than 52 years of age, were enrolled in this study. Uterosacral ligament (UL) biopsies were obtained from uterine specimens taken from 22 women with POP (n = 22, study group) and 34 myoma patients without POP (n = 34, control group) during abdominal or vaginal hysterectomy. Quantitative real-time polymerase chain reaction (Q-PCR) and immunohistochemistry analysis were applied in the present study.
The rate of high body mass index (BMI) (> 24 kg/m(2)) women was significantly higher in the POP group (81.8% vs. 35.3%, p = 0.001 *), and the BMI of the POP women was higher than that of the nonPOP women (p = 0.029 *). The mtDNA copy number (p = 0.001 *), collagen III alpha 1 (COL3α1) expression (p = 0.025 *), and MMP2 expression (p = 0.047 *) were significantly higher in the POP group when compared with the nonPOP group. The high BMI women had a higher mtDNA copy number (p = 0.002 *), COL3α1 (p = 0.028 *) gene expressions compared with the standard BMI women.
In the premenopausal state, higher BMI may be a stronger associate factor than vaginal birth for the development of POP. The higher mtDNA copy number, COL3α1, and MMP2 gene expressions are highly associated with POP in the UL of premenopausal women.
本研究旨在探讨绝经前女性子宫骶韧带中盆腔器官脱垂（POP）时线粒体DNA（mtDNA）拷贝数、胶原蛋白和基质金属蛋白酶（MMPs）表达的变化。
选取56名年龄均小于52岁的绝经前女性纳入本研究。在腹部或阴道子宫切除术中，从22例POP患者（n = 22，研究组）和34例无POP的肌瘤患者（n = 34，对照组）的子宫标本中获取子宫骶韧带（UL）活检组织。本研究采用定量实时聚合酶链反应（Q-PCR）和免疫组织化学分析。
POP组高体重指数（BMI）（> 24 kg/m²）女性的比例显著高于对照组（81.8% 对35.3%，p = 0.001*），且POP组女性的BMI高于非POP组女性（p = 0.029*）。与非POP组相比，POP组的mtDNA拷贝数（p = 0.001*）、胶原蛋白IIIα1（COL3α1）表达（p = 0.025*）和MMP2表达（p = 0.047*）显著更高。与标准BMI女性相比，高BMI女性的mtDNA拷贝数（p = 0.002*）、COL3α1（p = 0.028*）基因表达更高。
在绝经前状态下，较高的BMI可能是比阴道分娩更强的POP发生相关因素。较高的mtDNA拷贝数、COL3α1和MMP2基因表达与绝经前女性UL中的POP高度相关。
