Bortner C A, Arnold R R, Miller R D
Department of Microbiology and Immunology, School of Medicine, University of Louisville, KY 40292.
Can J Microbiol. 1989 Nov;35(11):1048-51. doi: 10.1139/m89-174.
Lactoferrin has been previously shown to be bactericidal for Legionella pneumophila. The current study showed that CaCl2, Mg(NO3)2, and MgCl2, but not NaCl, blocked killing. Activity was pH dependent with the greatest activity at 5.0. Sensitivity of the organism was dramatically affected by the growth conditions. Log phase 12 h, broth-grown cells were most sensitive, with older cultures becoming more resistant. Plate-grown cells were completely resistant. Lactoferrin binding, as detected by immunofluorescence microscopy, was temperature dependent (no binding at 4 degrees C), but was independent of killing.
乳铁蛋白先前已被证明对嗜肺军团菌具有杀菌作用。当前研究表明，氯化钙、硝酸镁和氯化镁可阻断杀菌作用，而氯化钠则不能。其活性依赖于pH值，在pH 5.0时活性最强。该生物体的敏感性受生长条件的显著影响。对数期12小时肉汤培养的细胞最为敏感，培养时间越长抗性越强。平板培养的细胞则完全具有抗性。通过免疫荧光显微镜检测发现，乳铁蛋白结合具有温度依赖性（4℃时无结合），但与杀菌作用无关。
