Zhang Yanhua, Ni Jian, Tang Fangping, Pei Kequan, Luo Yiqi, Jiang Lifen, Sun Lifu, Liang Yu
College of Life Sciences, Shaoxing University, Shaoxing, China.
State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
Sci Rep. 2016 Mar 1;6:22399. doi: 10.1038/srep22399.
Ericoid mycorrhiza (ERM) are expected to facilitate establishment of ericaceous plants in harsh habitats. However, diversity and driving factors of the root-associated fungi of ericaceous plants are poorly understood. In this study, hair-root samples of Vaccinium carlesii were taken from four forest types: old growth forests (OGF), secondary forests with once or twice cutting (SEC I and SEC II), and Cunninghamia lanceolata plantation (PLF). Fungal communities were determined using high-throughput sequencing, and impacts of human disturbances and the intra- and inter-annual variability of root-associated fungal community were evaluated. Diverse fungal taxa were observed and our results showed that (1) Intra- and inter-annual changes in root-associated fungal community were found, and the Basidiomycota to Ascomycota ratio was related to mean temperature of the sampling month; (2) Human disturbances significantly affected structure of root-associated fungal community of V. carlesii, and two secondary forest types were similar in root-associated fungal community and were closer to that of the old growth forest; (3) Plant community composition, edaphic parameters, and geographic factors significantly affected root-associated fungal communities of V. carlesii. These results may be helpful in better understanding the maintenance mechanisms of fungal diversity associated with hair roots of ERM plants under human disturbances.
预计欧石楠类菌根（ERM）有助于欧石楠科植物在恶劣生境中定植。然而，人们对欧石楠科植物根系相关真菌的多样性及其驱动因素了解甚少。在本研究中，从四种森林类型采集了江南越橘的须根样本：原始林（OGF）、经过一次或两次砍伐的次生林（SEC I和SEC II）以及杉木人工林（PLF）。使用高通量测序确定真菌群落，并评估人类干扰以及根系相关真菌群落的年内和年际变化的影响。观察到了多样的真菌分类群，我们的结果表明：（1）发现根系相关真菌群落存在年内和年际变化，担子菌门与子囊菌门的比例与采样月份的平均温度有关；（2）人类干扰显著影响江南越橘根系相关真菌群落的结构，两种次生林类型的根系相关真菌群落相似，且更接近原始林；（3）植物群落组成、土壤参数和地理因素显著影响江南越橘的根系相关真菌群落。这些结果可能有助于更好地理解在人类干扰下与ERM植物须根相关的真菌多样性的维持机制。
