检测恶性疟原虫环子孢子体抗体的酶联免疫吸附测定法。
ELISA method for detecting Plasmodium falciparum circumsporozoite antibody.
作者信息
Wirtz R A, Duncan J F, Njelesani E K, Schneider I, Brown A E, Oster C N, Were J B, Webster H K
出版信息
Bull World Health Organ. 1989;67(5):535-42.
An enzyme-linked immunosorbent assay (ELISA) for circulating IgG mouse antibody to Plasmodium falciparum circumsporozoite (CS) protein was modified for use with human sera collected in an area of northern Zambia that was endemic for malaria and from individuals never exposed to malaria. Optimum sensitivity was achieved using Immulon 2 microtitration plates, boiled casein-Tween 20 blocking buffer, and by adding a solution of boiled casein (4 micrograms/ml) to the capture antigen diluent. The results for the detection of anti-CS IgG correlated well with those of sporozoite immunofluorescence antibody assays. Modification of the ELISA method permitted the simultaneous detection of anti-CS IgG and IgM antibody on a single serum sample in the same well of the microtitration plate and the detection of anti-CS IgG antibody in Kenyan dried whole-blood samples collected on filter-paper. The assay has been used to monitor human antibody levels in a phase-I malaria vaccine trial and in longitudinal studies of malaria transmission in Thailand and Kenya.
一种用于检测循环中针对恶性疟原虫环子孢子（CS）蛋白的IgG小鼠抗体的酶联免疫吸附测定（ELISA）方法，被改良后用于检测在赞比亚北部疟疾流行地区采集的人类血清，以及从未接触过疟疾的个体的血清。使用Immulon 2微量滴定板、煮沸的酪蛋白 - 吐温20封闭缓冲液，并在捕获抗原稀释液中添加煮沸的酪蛋白溶液（4微克/毫升），可实现最佳灵敏度。抗CS IgG的检测结果与子孢子免疫荧光抗体测定结果高度相关。ELISA方法的改良使得能够在微量滴定板的同一孔中对单个血清样本同时检测抗CS IgG和IgM抗体，以及检测在滤纸上收集的肯尼亚干血样中的抗CS IgG抗体。该检测方法已用于在I期疟疾疫苗试验中监测人体抗体水平，以及在泰国和肯尼亚的疟疾传播纵向研究中。
Zotero 插件