Graczyk T K, Cranfield M R, Shiff C J
Johns Hopkins University School of Hygiene and Public Health, Department of Immunology and Infectious Diseases, Baltimore, MD 21205.
J Parasitol. 1993 Dec;79(6):879-85.
An enzyme-linked immunosorbent assay (ELISA) with 3 Plasmodium falciparum NF-54 antigens, R32tet32, P.F.R27, and crude red blood cell extract (CRBCE), was tested for detection of anti-Plasmodium relictum and anti-Plasmodium elongatum antibodies in sera from experimentally infected ducklings. Whole blood, serum, and dried blood on filter paper gave similar results. The latter was selected for convenience. All birds infected by experimental blood challenge, but not exposed to sporozoites, had detectable antibody (up to 1.0 x 10(-3.8) dilution) reactive with R32tet32, P.F.R27, and CRBCE antigens. Ducklings infected with P. elongatum had higher antibody levels than those infected with P. relictum. In a blind trial, the described ELISA accurately distinguished sera taken from infected and uninfected ducklings. This study provides the first evidence on cross reactivity in the ELISA format between P. falciparum antigens and antibodies induced by P. relictum and P. elongatum in experimentally infected ducklings. The proposed ELISA is fast, easy to perform, reproducible, and requires a minimal amount of equipment. The assay can be used for the detection of P. relictum and P. elongatum antibodies in captive or wild ducks, along with monitoring the level of antibody in selected groups of birds or for surveys of laboratory experiments where evidence of infection is required.
用3种恶性疟原虫NF-54抗原（R32tet32、P.F.R27和粗制红细胞提取物（CRBCE））进行酶联免疫吸附测定（ELISA），以检测实验感染雏鸭血清中的抗残疟原虫和抗延长疟原虫抗体。全血、血清和滤纸上的干血给出了相似的结果。出于方便起见，选择了后者。所有经实验性血液攻击感染但未接触子孢子的鸟类，其抗体（稀释度高达1.0×10(-3.8)）与R32tet32、P.F.R27和CRBCE抗原反应均可检测到。感染延长疟原虫的雏鸭抗体水平高于感染残疟原虫的雏鸭。在一项盲法试验中，所描述的ELISA能准确区分感染和未感染雏鸭的血清。本研究首次提供了在ELISA形式下，恶性疟原虫抗原与实验感染雏鸭中由残疟原虫和延长疟原虫诱导的抗体之间交叉反应性的证据。所提议的ELISA快速、易于操作、可重复，且所需设备最少。该检测方法可用于检测圈养或野鸭中的残疟原虫和延长疟原虫抗体，同时监测选定鸟类群体中的抗体水平，或用于需要感染证据的实验室实验调查。
