一种用于估算血清中抗惊厥药物游离水平的微量方法。

A micromethod for the estimation of free levels of anticonvulsant drugs in serum.

作者信息

Ratnaraj N, Goldberg V D, Hjelm M

机构信息

Department of Chemical Pathology, National Hospital for Nervous Diseases, London, UK.

出版信息

Clin Biochem. 1989 Dec;22(6):443-50. doi: 10.1016/s0009-9120(89)80096-7.

DOI:10.1016/s0009-9120(89)80096-7

PMID:2692873

Abstract

A micromethod for estimating free levels of phenobarbitone, phenytoin and carbamazepine in patients' sera is described. Serum samples are subjected to a process of ultrafiltration, the filtrates treated with acetonitrile and the drug concentration quantified using high performance liquid chromatography. The stability of free levels in specimens before and after storage is investigated. The method is reproducible and mean recovery exceeds 98.5% showing that there is no significant absorption of drug onto the filters used. There is no interference from other substances normally present in patients' sera and there is a good correlation between results obtained by this method and a fluorescence polarisation immunoassay with correlation coefficient between 0.975 and 0.999. Serum samples can be stored for a lengthy period before ultrafiltration without adverse effects. The relevance of the method to patient care is discussed.

摘要

描述了一种估算患者血清中苯巴比妥、苯妥英和卡马西平游离水平的微量方法。血清样本经过超滤过程，滤液用乙腈处理，并用高效液相色谱法定量药物浓度。研究了样本储存前后游离水平的稳定性。该方法具有可重复性，平均回收率超过98.5%，表明所用滤器对药物无明显吸附。患者血清中通常存在的其他物质无干扰，该方法与荧光偏振免疫分析法所得结果具有良好的相关性，相关系数在0.975至0.999之间。血清样本在超滤前可长时间储存而无不良影响。讨论了该方法与患者护理的相关性。