Fernandes Jason D, Booth David S, Frankel Alan D
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.
Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Wiley Interdiscip Rev RNA. 2016 Jul;7(4):470-86. doi: 10.1002/wrna.1342. Epub 2016 Mar 1.
HIV replication requires the nuclear export of essential, intron-containing viral RNAs. To facilitate export, HIV encodes the viral accessory protein Rev which binds unspliced and partially spliced viral RNAs and creates a ribonucleoprotein complex that recruits the cellular Chromosome maintenance factor 1 export machinery. Exporting RNAs in this manner bypasses the necessity for complete splicing as a prerequisite for mRNA export, and allows intron-containing RNAs to reach the cytoplasm intact for translation and virus packaging. Recent structural studies have revealed that this entire complex exhibits remarkable plasticity at many levels of organization, including RNA folding, protein-RNA recognition, multimer formation, and host factor recruitment. In this review, we explore each aspect of plasticity from structural, functional, and possible therapeutic viewpoints. WIREs RNA 2016, 7:470-486. doi: 10.1002/wrna.1342 For further resources related to this article, please visit the WIREs website.
HIV复制需要必需的、含内含子的病毒RNA进行核输出。为便于输出，HIV编码病毒辅助蛋白Rev，该蛋白与未剪接和部分剪接的病毒RNA结合，形成一种核糖核蛋白复合体，招募细胞染色体维持因子1输出机制。以这种方式输出RNA绕过了将完全剪接作为mRNA输出前提条件的必要性，并使含内含子的RNA完整地到达细胞质进行翻译和病毒包装。最近的结构研究表明，整个复合体在组织的许多层面都表现出显著的可塑性，包括RNA折叠、蛋白质-RNA识别、多聚体形成和宿主因子招募。在这篇综述中，我们从结构、功能和可能的治疗观点探讨可塑性的各个方面。WIREs RNA 2016, 7:470-486。doi: 10.1002/wrna.1342 有关本文的更多资源，请访问WIREs网站。
