Poulsen O M, Jacobsen T, Hau J, Jensen B, Vodder L, Andersen F
Department of Veterinary Pathology, Veterinary and Agricultural University, Copenhagen, Denmark.
Electrophoresis. 1989 Dec;10(12):857-64. doi: 10.1002/elps.1150101211.
The development of rocket enzyme activity electrophoresis for the detection and quantification of various proteinases, lipases and pectinases is presented. Rocket enzyme activity electrophoresis is more sensitive than the radial diffusion assay and often enables distinction between qualitatively different enzymes present in the same samples, whereas the radial diffusion assay only provides information on the overall enzyme activity. However, calibration and optimization of the enzyme activity electrophoretic assay have to be performed for each new enzyme-substrate system to be analyzed. Some of the common pitfalls in the development of new enzyme activity electrophoretic assays are presented. Enzyme activity electrophoresis can be applied in combination with other electrophoretic assays. Particularly the combination of enzyme activity electrophoresis with various immunoelectrophoretic methods can provide detailed information on the enzymes studies.
本文介绍了用于检测和定量各种蛋白酶、脂肪酶和果胶酶的火箭酶活性电泳技术的发展。火箭酶活性电泳比径向扩散测定法更灵敏，通常能够区分同一样品中性质不同的酶，而径向扩散测定法仅提供关于总酶活性的信息。然而，对于每一种要分析的新酶 - 底物系统，都必须进行酶活性电泳测定的校准和优化。文中还介绍了新酶活性电泳测定法开发过程中一些常见的陷阱。酶活性电泳可与其他电泳测定法联合应用。特别是酶活性电泳与各种免疫电泳方法的结合，可以提供有关所研究酶的详细信息。
