Jacobsen T, Poulsen O M, Hau J
Department of General Microbiology, University of Copenhagen, Denmark.
Electrophoresis. 1989 Jan;10(1):49-52. doi: 10.1002/elps.1150100112.
The development and application of a rocket immunoelectrophoretic and an enzyme activity electrophoretic assay for the qualitative analysis of Geotrichum candidum lipase activity is presented. The sensitivities of the four assays were (in arbitrary units): enzyme activity electrophoresis, 1-0.5; rocket immunoelectrophoresis, 0.5-0.2; radial diffusion, 1; titrimetry, 1. The electrophoretic methods made it possible to distinguish between high and low molecular weight forms of the G. candidum lipases. The enzyme activity electrophoretic methods can be combined with other electrophoretic techniques, as demonstrated here with isoelectric focusing, and produce useful information on physico-chemical differences between different molecular forms of the lipase, e.g. forms with different pI.
本文介绍了用于白地霉脂肪酶活性定性分析的火箭免疫电泳法和酶活性电泳法的开发与应用。四种测定方法的灵敏度（以任意单位计）分别为：酶活性电泳法为1 - 0.5；火箭免疫电泳法为0.5 - 0.2；径向扩散法为1；滴定法为1。电泳方法能够区分白地霉脂肪酶的高分子量和低分子量形式。如本文通过等电聚焦所证明的，酶活性电泳方法可与其他电泳技术相结合，从而获得关于脂肪酶不同分子形式之间物理化学差异的有用信息，例如具有不同pI的形式。
