Pantazopoulou Areti
Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, Madrid 28040, Spain
Mycologia. 2016 May-Jun;108(3):603-22. doi: 10.3852/15-309. Epub 2016 Mar 1.
Cargo passage through the Golgi, albeit an undoubtedly essential cellular function, is a mechanistically unresolved and much debated process. Although the main molecular players are conserved, diversification of the Golgi among different eukaryotic lineages is providing us with tools to resolve standing controversies. During the past decade the Golgi apparatus of model filamentous fungi, mainly Aspergillus nidulans, has been intensively studied. Here an overview of the most important findings in the field is provided. Golgi architecture and dynamics, as well as the novel cell biology tools that were developed in filamentous fungi in these studies, are addressed. An emphasis is placed on the central role the Golgi has as a crossroads in the endocytic and secretory-traffic pathways in hyphae. Finally the major advances that the A. nidulans Golgi biology has yielded so far regarding our understanding of key Golgi regulators, such as the Rab GTPases RabC(Rab6) and RabE(Rab11), the oligomeric transport protein particle, TRAPPII, and the Golgi guanine nucleotide exchange factors of Arf1, GeaA(GBF1/Gea1) and HypB(BIG/Sec7), are highlighted.
尽管货物通过高尔基体无疑是一项至关重要的细胞功能，但这一过程在机制上尚未得到解决，且存在诸多争议。虽然主要的分子参与者是保守的，但不同真核生物谱系中高尔基体的多样化为我们提供了解决现有争议的工具。在过去十年中，对模式丝状真菌（主要是构巢曲霉）的高尔基体进行了深入研究。本文提供了该领域最重要发现的概述。文中讨论了高尔基体的结构和动力学，以及在这些研究中丝状真菌开发的新型细胞生物学工具。重点强调了高尔基体在菌丝内吞和分泌运输途径中作为交叉点的核心作用。最后，突出了构巢曲霉高尔基体生物学在我们对关键高尔基体调节因子（如Rab GTPases RabC（Rab6）和RabE（Rab11）、寡聚运输蛋白颗粒TRAPPII以及Arf1的高尔基体鸟嘌呤核苷酸交换因子GeaA（GBF1/Gea1）和HypB（BIG/Sec7））的理解方面迄今所取得的主要进展。
