Al-Dhabi Naif Abdullah, Valan Arasu Mariadhas
Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Evid Based Complement Alternat Med. 2016;2016:7631864. doi: 10.1155/2016/7631864. Epub 2016 Jan 4.
The present study aimed to profile the polyunsaturated fatty acids, sugars, free amino acids, and polyphenols in 37 varieties of Spirulina commonly available in the market using gas chromatography and high performance liquid chromatography. In addition, the biological potentials of the Spirulina samples were evaluated by analysing the in vitro antioxidant activities using various analytical techniques. The analyses revealed the presence of 13 polyunsaturated fatty acids, 18 amino acids, 7 sugars, and polyphenols. The polyunsaturated fatty acids contents were varied between Spirulina samples. The total polyunsaturated fatty acids amount was 4.25 mg/100 g, and the average among of sapienic acid detected was 2.25 mg/100 g, which was followed by linoleic acid (16.7%) and γ-linolenic acid (14%). Among the 7 sugars, the hexose levels were the highest (73.85%). The total amino acids contents ranged from 11.49 to 56.14 mg/100 g, and the individual essential amino acids accounted for 17% to 39.18%. The "natural" tablets exhibited the highest polyphenols levels (24 mg/g). All of the Spirulina samples expressed dose-dependent antioxidant activities. The polyunsaturated fatty acids, sugars, free amino acids, and polyphenols contents varied widely, and the variations in these compounds between the Spirulina samples were significant.
本研究旨在利用气相色谱法和高效液相色谱法对市场上常见的37种螺旋藻中的多不饱和脂肪酸、糖类、游离氨基酸和多酚进行分析。此外,通过使用各种分析技术分析体外抗氧化活性,对螺旋藻样品的生物活性进行了评估。分析结果显示,螺旋藻样品中存在13种多不饱和脂肪酸、18种氨基酸、7种糖类和多酚。螺旋藻样品中的多不饱和脂肪酸含量各不相同。多不饱和脂肪酸总量为4.25毫克/100克,检测到的鲨油酸平均含量为2.25毫克/100克,其次是亚油酸(16.7%)和γ-亚麻酸(14%)。在7种糖类中,己糖水平最高(73.85%)。总氨基酸含量在11.49至56.14毫克/100克之间,个别必需氨基酸占17%至39.18%。“天然”片剂的多酚含量最高(24毫克/克)。所有螺旋藻样品均表现出剂量依赖性抗氧化活性。多不饱和脂肪酸、糖类、游离氨基酸和多酚含量差异很大,螺旋藻样品之间这些化合物的差异显著。