Tummala Shashank, Gowthamarajan K, Satish Kumar M N
a Department of Pharmaceutics , J. S. S. College of Pharmacy (off-Campus), J. S. S University , Mysore , Karnataka , India.
b Department of Pharmacology , J. S. S. College of Pharmacy (off-Campus), J. S. S University , Mysore , Karnataka , India.
Artif Cells Nanomed Biotechnol. 2017 Mar;45(2):261-269. doi: 10.3109/21691401.2016.1146730. Epub 2016 Mar 2.
In the present study, we have investigated the enhanced synergistic and apoptotic activity of immunohybrid nanoparticles encapsulating oxaliplatin and covalently conjugated with TRAIL (Apo-2L/CD-253). Time-dependent cytotoxicity activity of nanoparticles was determined by MTT assay in HT-29 cells. Nuclear morphological changes and assessment of apoptotic ratio was analyzed by DAPI (4'6-diamidino-2-phenylindole) staining and annexin-propidium iodide (PI) assay. Cell-cycle analysis of oxaliplatin in HT-29 cell was analyzed by flow cytometry at 72 h. Furthermore, molecular mechanisms related to oxaliplatin-induced anticancer activity was explored by western blot analysis. Our study revealed appreciable time-dependent cytotoxicity, apoptotic, and synergistic activity of oxaliplatin immunohybrid nanoparticles.
在本研究中，我们研究了包裹奥沙利铂并与TRAIL（凋亡素-2配体/CD-253）共价偶联的免疫杂交纳米颗粒增强的协同和凋亡活性。通过MTT法在HT-29细胞中测定纳米颗粒的时间依赖性细胞毒性活性。通过4',6-二脒基-2-苯基吲哚（DAPI）染色和膜联蛋白-碘化丙啶（PI）测定法分析核形态变化和凋亡率评估。在72小时时通过流式细胞术分析HT-29细胞中奥沙利铂的细胞周期。此外，通过蛋白质印迹分析探索了与奥沙利铂诱导的抗癌活性相关的分子机制。我们的研究揭示了奥沙利铂免疫杂交纳米颗粒明显的时间依赖性细胞毒性、凋亡和协同活性。
