Souza E A, Zaluski R, Veiga N, Orsi R O
Departamento de Produção Animal, Universidade Estadual Paulista, Botucatu, SP, Brazil.
Departamento de Produção e Exploração, Universidade Estadual Paulista, Botucatu, SP, Brazil.
Braz J Biol. 2016 Jun;76(2):396-401. doi: 10.1590/1519-6984.16714. Epub 2016 Mar 1.
The effects of seasonal variations and the methods of collection of propolis produced by Africanized honey bees Apis mellifera Linnaeus, 1758, on the composition of constituent minerals such as magnesium (Mg), zinc (Zn), iron (Fe), sodium (Na), calcium (Ca), copper (Cu), and potassium (K) were evaluated. Propolis was harvested from 25 beehives by scraping or by means of propolis collectors (screen, "intelligent" collector propolis [ICP], lateral opening of the super [LOS], and underlay method). During the one-year study, the propolis produced was harvested each month, ground, homogenized, and stored in a freezer at -10 ºC. Seasonal analyses of the mineral composition were carried out by atomic absorption spectrophotometry and the results were evaluated by analysis of variance (ANOVA), followed by Tukey-Kramer's test to compare the mean values (p<0.05). The results showed that seasonal variations influence the contents of 5 minerals (Mg, Fe, Na, Ca, and Cu), and the propolis harvesting method affects the contents of 4 minerals (Mg, Zn, Fe, and Ca).
评估了季节变化以及1758年林奈氏意大利蜜蜂（Apis mellifera Linnaeus）所产蜂胶的采集方法对镁（Mg）、锌（Zn）、铁（Fe）、钠（Na）、钙（Ca）、铜（Cu）和钾（K）等组成矿物质成分的影响。通过刮取或使用蜂胶采集器（筛网、“智能”蜂胶采集器[ICP]、继箱侧开口[LOS]和衬垫法）从25个蜂箱中采集蜂胶。在为期一年的研究中，每月采集所产蜂胶，研磨、均质化后储存在-10℃的冰箱中。通过原子吸收分光光度法对矿物质成分进行季节性分析，并通过方差分析（ANOVA）评估结果随后进行Tukey-Kramer检验以比较平均值（p<0.05）。结果表明，季节变化影响5种矿物质（Mg、Fe、Na、Ca和Cu）的含量，蜂胶采集方法影响4种矿物质（Mg、Zn、Fe和Ca）的含量。
