Rizzo Roberta, Bergamini Gabriella, Bortolotti Daria, Leal Teresinha, D'Orazio Ciro, Pintani Emily, Melchiorri Loredana, Zavatti Eleonora, Assael Baroukh M, Sorio Claudio, Melotti Paola
Section of Microbiology & Medical Genetics, Medical Sciences Department, Ferrara University, 44121 Ferrara FE, Italy.
Department of Medicine, Cystic Fibrosis Translational Research Laboratory "Daniele Lissandrini," University of Verona, Verona, Italy.
Future Microbiol. 2016;11(3):363-73. doi: 10.2217/fmb.15.143. Epub 2016 Mar 2.
Deregulated immune response fails to control biofilm-forming bacteria, as Pseudomonas aeruginosa, in the lungs of cystic fibrosis (CF) patients. HLA-G is an immune-modulatory molecule involved in respiratory diseases and infections.
MATERIALS & METHODS: HLA-G mRNA and protein were analyzed in plasma and exhaled breath condensate from CF patients undergoing intravenous antibiotic treatment, CF cell line and murine model.
Therapy normalizes HLA-G plasmatic in CF patients suggesting a systemic anti-inflammatory role while in CF airway system, higher expression of HLA-G is associated with P. aeruginosa infection. CF cell line and murine model expressed higher HLA-G molecules in the presence of P. aeruginosa.
Plasmatic and lung HLA-G expression suggest a role in reducing systemic inflammation and supporting P. aeruginosa infection.
免疫反应失调无法控制囊性纤维化（CF）患者肺部形成生物膜的细菌，如铜绿假单胞菌。HLA - G是一种参与呼吸系统疾病和感染的免疫调节分子。
对接受静脉抗生素治疗的CF患者的血浆和呼出气冷凝物、CF细胞系和小鼠模型中的HLA - G mRNA和蛋白进行分析。
治疗使CF患者的HLA - G血浆水平正常化，提示其具有全身抗炎作用，而在CF气道系统中，HLA - G的高表达与铜绿假单胞菌感染相关。在有铜绿假单胞菌存在的情况下，CF细胞系和小鼠模型表达更高的HLA - G分子。
血浆和肺部HLA - G表达提示其在减轻全身炎症和支持铜绿假单胞菌感染方面发挥作用。
