囊性纤维化患者感染铜绿假单胞菌期间HLA - G的表达与调控
HLA-G expression and regulation during Pseudomonas aeruginosa infection in cystic fibrosis patients.
作者信息
Rizzo Roberta, Bergamini Gabriella, Bortolotti Daria, Leal Teresinha, D'Orazio Ciro, Pintani Emily, Melchiorri Loredana, Zavatti Eleonora, Assael Baroukh M, Sorio Claudio, Melotti Paola
机构信息
Section of Microbiology & Medical Genetics, Medical Sciences Department, Ferrara University, 44121 Ferrara FE, Italy.
Department of Medicine, Cystic Fibrosis Translational Research Laboratory "Daniele Lissandrini," University of Verona, Verona, Italy.
出版信息
Future Microbiol. 2016;11(3):363-73. doi: 10.2217/fmb.15.143. Epub 2016 Mar 2.
BACKGROUND
Deregulated immune response fails to control biofilm-forming bacteria, as Pseudomonas aeruginosa, in the lungs of cystic fibrosis (CF) patients. HLA-G is an immune-modulatory molecule involved in respiratory diseases and infections.
MATERIALS & METHODS: HLA-G mRNA and protein were analyzed in plasma and exhaled breath condensate from CF patients undergoing intravenous antibiotic treatment, CF cell line and murine model.
RESULTS
Therapy normalizes HLA-G plasmatic in CF patients suggesting a systemic anti-inflammatory role while in CF airway system, higher expression of HLA-G is associated with P. aeruginosa infection. CF cell line and murine model expressed higher HLA-G molecules in the presence of P. aeruginosa.
CONCLUSION
Plasmatic and lung HLA-G expression suggest a role in reducing systemic inflammation and supporting P. aeruginosa infection.
背景
免疫反应失调无法控制囊性纤维化（CF）患者肺部形成生物膜的细菌，如铜绿假单胞菌。HLA - G是一种参与呼吸系统疾病和感染的免疫调节分子。
材料与方法
对接受静脉抗生素治疗的CF患者的血浆和呼出气冷凝物、CF细胞系和小鼠模型中的HLA - G mRNA和蛋白进行分析。
结果
治疗使CF患者的HLA - G血浆水平正常化，提示其具有全身抗炎作用，而在CF气道系统中，HLA - G的高表达与铜绿假单胞菌感染相关。在有铜绿假单胞菌存在的情况下，CF细胞系和小鼠模型表达更高的HLA - G分子。
结论
血浆和肺部HLA - G表达提示其在减轻全身炎症和支持铜绿假单胞菌感染方面发挥作用。
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