泰国偶蹄目动物蜱中里氏杆菌、无形体、柯克斯体和弗朗西斯菌属细菌的分子检测
Molecular detection of Rickettsia, Anaplasma, Coxiella and Francisella bacteria in ticks collected from Artiodactyla in Thailand.
作者信息
Sumrandee Chalao, Baimai Visut, Trinachartvanit Wachareeporn, Ahantarig Arunee
机构信息
Biodiversity Research Cluster, Department of Biology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand; Department of Fundamental Science, Faculty of Science and Technology, Surindra Rajabhat University, Muang District, Surin 32000, Thailand.
Biodiversity Research Cluster, Department of Biology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand; Center of Excellence for Vectors and Vector-Borne Diseases, Faculty of Science, Mahidol University at Salaya, Phutthamonthon 4 Road, Nakhon Pathom 73170, Thailand; Department of Fundamental Science, Faculty of Science and Technology, Surindra Rajabhat University, Muang District, Surin 32000, Thailand.
出版信息
Ticks Tick Borne Dis. 2016 Jul;7(5):678-689. doi: 10.1016/j.ttbdis.2016.02.015. Epub 2016 Feb 23.
A total of 79 ticks collected from Sambar deer (Cervus unicolor), Barking deer (Muntiacus muntjak) and Wild boar (Sus scrofa) were examined by PCR for the presence of Rickettsia, Anaplasma, Coxiella, and Francisella bacteria. Of the 79 ticks, 13% tested positive for Rickettsia, 15% tested positive for Anaplasma, 4% tested positive for Coxiella, and 3% tested positive for Francisella. Interestingly, triple infection with Anaplasma, Rickettsia and Francisella was determined in a Dermacentor auratus tick. Moreover, another triple infection with Rickettsia, Anaplasma, and Coxiella was found in a Haemaphysalis lagrangei tick. Double infection of Rickettsia with Coxiella was also detected in another H. lagrangei tick. From the phylogenetic analyses, we found a Rickettsia sp. with a close evolutionary relationship to Rickettsia bellii in the H. lagrangei tick. We also found the first evidence of a Rickettsia sp. that is closely related to Rickettsia tamurae in Rhipicephalus (Boophilus) microplus ticks from Thailand. H. lagrangei and Haemaphysalis obesa ticks collected from Sambar deer tested positive for Anaplasma species form the same clade with Anaplasma bovis. In contrast, other H. lagrangei ticks collected from Sambar deer and D. auratus ticks collected from Wild boar were also reported for the first time to be infected with an Anaplasma species that is closely related to Anaplasma platys. The phylogenetic analysis of the 16S rRNA gene of Coxiella bacteria revealed that Coxiella symbionts from H. lagrangei formed a distinctly different lineage from Coxiella burnetii (a human pathogen). Additionally, Francisella bacteria identified in D. auratus ticks were found to be distantly related to a group of pathogenic Francisella species. The identification of these bacteria in several feeding ticks suggests the risk of various emerging tick-borne diseases and endosymbionts in humans, wildlife, and domestic animals in Thailand.
从水鹿（Cervus unicolor）、赤麂（Muntiacus muntjak）和野猪（Sus scrofa）身上采集了79只蜱虫，通过聚合酶链反应（PCR）检测其中是否存在立克次氏体、无形体、柯克斯体和弗朗西斯菌属细菌。在这79只蜱虫中，13%的蜱虫立克次氏体检测呈阳性，15%的蜱虫无形体检测呈阳性，4%的蜱虫柯克斯体检测呈阳性，3%的蜱虫弗朗西斯菌属检测呈阳性。有趣的是，在一只长角血蜱（Dermacentor auratus）中发现了无形体、立克次氏体和弗朗西斯菌属的三重感染。此外，在一只拉氏血蜱（Haemaphysalis lagrangei）中发现了另一起立克次氏体、无形体和柯克斯体的三重感染。在另一只拉氏血蜱中还检测到立克次氏体与柯克斯体的双重感染。通过系统发育分析，我们在拉氏血蜱中发现了一种与贝利氏立克次氏体（Rickettsia bellii）进化关系密切的立克次氏体菌株。我们还首次发现了在泰国微小牛蜱（Rhipicephalus (Boophilus) microplus）中存在一种与田村立克次氏体（Rickettsia tamurae）密切相关的立克次氏体菌株的证据。从水鹿身上采集的拉氏血蜱和肥胖血蜱（Haemaphysalis obesa）中检测到的无形体物种与牛无形体（Anaplasma bovis）属于同一进化枝。相比之下，从水鹿身上采集的其他拉氏血蜱以及从野猪身上采集的长角血蜱也首次被报道感染了一种与血小板无形体（Anaplasma platys）密切相关的无形体物种。对柯克斯体细菌的16S rRNA基因进行系统发育分析表明，来自拉氏血蜱的柯克斯体共生菌与贝氏柯克斯体（Coxiella burnetii，一种人类病原体）形成了明显不同的谱系。此外，在长角血蜱中鉴定出的弗朗西斯菌属细菌与一组致病性弗朗西斯菌物种的亲缘关系较远。在几只吸食血液的蜱虫中鉴定出这些细菌，表明泰国的人类、野生动物和家畜面临着各种新出现的蜱传疾病和内共生菌的风险。
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