Riis B, Rattan S I, Clark B F
Department of Chemistry, Aarhus University, Denmark.
J Biochem Biophys Methods. 1989 Oct;19(4):319-25. doi: 10.1016/0165-022x(89)90063-8.
The content of the elongation factor (EF-2) can be measured by diphtheria toxin-dependent ADP-ribosylation in cell-free extracts of samples prepared from small amounts of tissues and cells containing less than 100 micrograms of total protein. A 20 min in vitro assay, in which a radioactive ADP-ribosyl residue is transferred specifically and 1:1 stoichiometrically to EF-2, is sufficient to estimate the total amounts of ADP-ribosylatable active EF-2. The method is very useful for monitoring changing levels of EF-2 during various pathological and biological processes, including cell cycle, ageing, cancer and other diseases.
延伸因子（EF-2）的含量可通过在含有少于100微克总蛋白的少量组织和细胞制备的样品的无细胞提取物中，用依赖白喉毒素的ADP-核糖基化来测量。一个20分钟的体外测定，其中放射性ADP-核糖基残基以1:1的化学计量比特异性转移到EF-2上，足以估计可进行ADP-核糖基化的活性EF-2的总量。该方法对于监测各种病理和生物学过程（包括细胞周期、衰老、癌症和其他疾病）中EF-2水平的变化非常有用。
