Aissa A, Manolaraki F, Ben Salem H, Hoste H, Kraiem K
Institut Supérieur Agronomique de Chott-Mariem - Université de Sousse,BP 47,4042 Chott-Mariem,Tunisie.
UMR 1225 INRA/ENVT, Ecole Nationale Vétérinaire de Toulouse - 23 Chemin des Capelles,31076 Toulouse Cedex,France.
Parasitology. 2016 May;143(6):778-86. doi: 10.1017/S0031182016000317. Epub 2016 Mar 3.
Gastrointestinal nematodes are compromising productivity of grazing sheep and goats. Therefore, scientists have been looking for cost-effective alternative options. Forage legumes (Fabacea Family) contain tannins that could improve livestock performance and their health as well. The present study aimed to (i) determine the in vitro anthelmintic (AH) activity of 19 acetonic extracts of Hedysarum carnosum Desf on Haemonchus contortus by a larval exsheathment assay (LEA); (ii) test the anthelmintic activity of condensed tannins using a deactivating reagent, polyvinylpolypyrrolidone (PVPP); (iii) study the effect of location and the phenological stage on the percentage of exsheathment. The LEA was used at different concentrations (150, 300, 600, 1200 µg mL-1 of acetonic extract/mL of purified buffer solution (PBS)). The larval exsheathment is concentration, location, phenological stage dependent. All extracts, caused a delay of the percentage of exsheathment over 50% so the AH activity of H. carnosum was confirmed. After addition of PVPP, the % exsheathment was similar to the 150 µg mL-1 concentration. The biplot showed that Loc1(S), Loc4(B), Loc 5(PF), Loc 6(BM) and Loc 6(PF) were isolated from other plant extract sample. Our in vitro study showed that H. carnosum seems to be a promising alternative to AH drugs.
胃肠道线虫正影响着放牧绵羊和山羊的生产性能。因此，科学家们一直在寻找经济有效的替代方案。豆科牧草含有单宁，可提高家畜的生产性能并改善其健康状况。本研究旨在：（i）通过幼虫脱鞘试验（LEA）测定19种肉苁蓉丙酮提取物对捻转血矛线虫的体外驱虫活性；（ii）使用失活剂聚乙烯聚吡咯烷酮（PVPP）测试缩合单宁的驱虫活性；（iii）研究位置和物候期对脱鞘百分比的影响。LEA在不同浓度（150、300、600、1200 µg/mL丙酮提取物/纯化缓冲溶液（PBS））下进行。幼虫脱鞘取决于浓度、位置和物候期。所有提取物均使脱鞘百分比延迟超过50%，因此证实了肉苁蓉的驱虫活性。添加PVPP后，脱鞘百分比与150 µg/mL浓度时相似。双标图显示，Loc1（S）、Loc4（B）、Loc 5（PF）、Loc 6（BM）和Loc 6（PF）与其他植物提取物样本分离。我们的体外研究表明，肉苁蓉似乎是驱虫药物的一个有前景的替代品。
