在两名来自墨西哥城且先前已接种疫苗的患者中检测到腮腺炎病毒H基因型。

Detection of mumps virus genotype H in two previously vaccinated patients from Mexico City.

作者信息

Del Valle Alberto, García Alí A, Barrón Blanca L

机构信息

Laboratory of Virology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala S/N, Casco de Santo Tomás, 11340, Mexico, D.F., Mexico.

出版信息

Arch Virol. 2016 Jun;161(6):1639-44. doi: 10.1007/s00705-016-2770-6. Epub 2016 Mar 3.

DOI:10.1007/s00705-016-2770-6

PMID:26935913

Abstract

Infections caused by mumps virus (MuV) have been successfully prevented through vaccination; however, in recent years, an increasing number of mumps outbreaks have been reported within vaccinated populations. In this study, MuV was genotyped for the first time in Mexico. Saliva samples were obtained from two previously vaccinated patients in Mexico City who had developed parotitis. Viral isolation was carried out in Vero cells, and the SH and HN genes were amplified by RT-PCR. Amplicons were sequenced and compared to a set of reference sequences to identify the MuV genotype.

摘要

通过接种疫苗已成功预防了由腮腺炎病毒（MuV）引起的感染；然而，近年来，在已接种疫苗的人群中报告的腮腺炎疫情越来越多。在本研究中，首次在墨西哥对MuV进行了基因分型。从墨西哥城两名先前接种过疫苗且患腮腺炎的患者身上采集了唾液样本。在Vero细胞中进行病毒分离，并通过RT-PCR扩增SH和HN基因。对扩增子进行测序，并与一组参考序列进行比较以鉴定MuV基因型。