Li L, Zhao D, Chen D F, Zhang E S, Guo S Y
Department of Surgical Osteoarthritis, Jinan Central Hospital Affiliated to Shandong University, No. 105 Jiefang Road Lixia District, Jinan, 250013, China.
Department of Pathology, Jinan Central Hospital, Jinan, 250013, China.
Eur J Trauma Emerg Surg. 2017 Apr;43(2):249-253. doi: 10.1007/s00068-016-0648-7. Epub 2016 Mar 2.
To provide experimental basis for clinic treatment by observing the effects of simple acute hematoma on cartilage and synovium, and improvement of hyaluronic acid (HA) on the damage.
Twenty-four New Zealand white rabbits were randomly divided 3 groups according to killed time: hematoma group, HA group and control group. These rabbits were killed at 4 days, 2, 4 and 8 weeks, respectively.
Knee articular cartilage and synovium were obtained to detect histology, histochemistry and chondroitin proteoglycans (PG). Morphologies of cartilage and synovium in the control group were normal, while cartilage cells were detected hyperplasia of activation and each layer was disorganized in hematoma groups with 4 days and 2 weeks. Compared to the control group, concentrations of PG in 4 days and 2 weeks groups were significantly reduced (p < 0.05), while no significant difference was detected between 4 weeks group and the control group. Morphology was recovered normal in 8 weeks. Compared to hematoma group, morphology and histochemistry were improved in HA group at each time frame.
Simple acute hematoma can lead to temporary change of morphology and biochemistry. Application of HA can reduce damage of hemarthrosis on cartilaginous and shorten the cartilage recovery time.
通过观察单纯急性血肿对软骨和滑膜的影响以及透明质酸（HA）对损伤的改善作用，为临床治疗提供实验依据。
将24只新西兰白兔按处死时间随机分为3组：血肿组、HA组和对照组。分别于4天、2周、4周和8周处死这些兔子。
获取膝关节软骨和滑膜进行组织学、组织化学及软骨素蛋白聚糖（PG）检测。对照组软骨和滑膜形态正常，而血肿组4天和2周时软骨细胞出现活化增生，各层排列紊乱。与对照组相比，4天和2周组的PG浓度显著降低（p<0.05），而4周组与对照组之间未检测到显著差异。8周时形态恢复正常。与血肿组相比，HA组在各时间点的形态和组织化学均有所改善。
单纯急性血肿可导致形态和生化的暂时改变。应用HA可减轻关节积血对软骨的损伤并缩短软骨恢复时间。
