Zhang Heng-Xi, Zhao Jin-Xin, Chen Guan-Jun, Du Zong-Jun
College of Marine Science, Shandong University at Weihai, Weihai, 264209, People's Republic of China.
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
Antonie Van Leeuwenhoek. 2016 May;109(5):705-11. doi: 10.1007/s10482-016-0671-z. Epub 2016 Mar 2.
A marine bacterium, designated strain N39(T), was isolated from a sediment sample collected at a sea cucumber culture pond in Weihai, China. Cells of strain N39(T) were observed to be Gram-stain negative, facultatively anaerobic, motile rods showing catalase and oxidase negative reactions. Strain N39(T) was found to grow optimally at pH 8.0-8.5, 35-37 °C and in the presence of approximately 3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain N39(T) belongs to the genus Kordiimonas in the family Kordiimonadaceae, appearing closely related to Kordiimonas lacus JCM 16261(T) (95.9 %), Kordiimonas aquimaris MEBiC06554(T) (95.1 %), Kordiimonas gwangyangensis JCM 12864(T) (94.2 %) and Kordiimonas aestuarii 101-1(T) (93.8 %). Ubiquinone 10 (Q-10) was found to be the major respiratory quinone. The dominant cellular fatty acids were identified as iso-C17:1 ω9c, iso-C17:0, iso-C15:0 and C17:1 ω6c. The predominant polar lipids were found to be phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. The DNA G + C content of strain N39(T) is 50.8 %. On the basis of genotypic, chemotaxonomic and phenotypic data, strain N39(T) is concluded to represent a novel species within the genus Kordiimonas, for which the name Kordiimonas sediminis sp. nov. is proposed. The type strain is N39(T) (=KCTC 42590(T) = MCCC 1H00112(T)).
从中国威海海参养殖池塘采集的沉积物样本中分离出一株海洋细菌，命名为菌株N39(T)。观察到菌株N39(T)细胞革兰氏染色呈阴性，兼性厌氧，为运动性杆菌，过氧化氢酶和氧化酶反应呈阴性。发现菌株N39(T)在pH 8.0 - 8.5、35 - 37°C和约3.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株N39(T)属于科氏单胞菌科的科氏单胞菌属，与湖泊科氏单胞菌JCM 16261(T)（95.9%）、海洋科氏单胞菌MEBiC06554(T)（95.1%）、光阳科氏单胞菌JCM 12864(T)（94.2%）和河口科氏单胞菌101 - 1(T)（93.8%）密切相关。发现泛醌10（Q - 10）是主要的呼吸醌。主要的细胞脂肪酸被鉴定为异-C17:1 ω9c、异-C17:0、异-C15:0和C17:1 ω6c。发现主要的极性脂为磷脂酰甘油、磷脂酰乙醇胺和二磷脂酰甘油。菌株N39(T)的DNA G + C含量为50.8%。基于基因型、化学分类和表型数据，得出菌株N39(T)代表科氏单胞菌属内一个新物种的结论，为此提出新名称海洋科氏单胞菌（Kordiimonas sediminis sp. nov.）。模式菌株为N39(T)（=KCTC 42590(T) = MCCC 1H00112(T)）。
