Han Ji-Ru, Fang Dong-Bo, Xia Hai-Feng, Chen Guan-Jun, Du Zong-Jun
College of Marine Science, Shandong University, Weihai 264209, PR China.
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1932-1936. doi: 10.1099/ijsem.0.001889. Epub 2017 Jun 9.
A Gram-stain-negative, non-motile, rod-shaped, red-pigmented, facultatively anaerobic bacterium, designated SS2-9T, was isolated from sediment collected from a sea cucumber culture pond located in Rongcheng, Shandong province, China. Cells of strain SS2-9T were approximately 0.3-0.5 µm in width and 1.5-6.0 µm in length. The strain was able to grow at 10-37 °C, at pH 6.5-8.5 and in the presence of 0.5-6.0 % (w/v) NaCl. It grew optimally at 28 °C and in the presence of 2.0 % (w/v) NaCl. The DNA G+C content was 34.5 mol% and the sole respiratory quinone was menaquinone 6 (MK-6). The predominant cellular fatty acids were C15 : 0, iso-C15 : 1 G, iso-C15 : 0 and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, an unidentified phospholipid, two unidentified aminolipids and four unidentified lipids. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SS2-9T was phylogenetically related to members of the genus Aquimarina and was closely related to Aquimarina amphilecti 92VT (97.29 % similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain SS2-9T was considered to represent a novel species of the genus Aquimarina, for which the name Aquimarina rubra sp. nov. is proposed. The type strain is SS2-9T (=KCTC 52274T=MCCC 1H00142T).
从中国山东省荣成市海参养殖池塘采集的沉积物中分离出一株革兰氏阴性、无运动性、杆状、红色色素、兼性厌氧细菌，命名为SS2-9T。SS2-9T菌株的细胞宽度约为0.3-0.5μm，长度为1.5-6.0μm。该菌株能够在10-37°C、pH 6.5-8.5以及0.5-6.0%（w/v）NaCl存在的条件下生长。在28°C和2.0%（w/v）NaCl存在的条件下生长最佳。DNA G+C含量为34.5mol%，唯一的呼吸醌是甲基萘醌6（MK-6）。主要的细胞脂肪酸为C15:0、异C15:1 G、异C15:0和异C17:0 3-OH。主要的极性脂为磷脂酰乙醇胺、一种未鉴定的磷脂、两种未鉴定的氨基脂和四种未鉴定的脂。基于16S rRNA基因序列的系统发育分析表明，SS2-9T菌株与海栖菌属的成员在系统发育上相关，并且与嗜礁海栖菌92VT密切相关（相似性为97.29%）。基于表型、化学分类和系统发育数据，SS2-9T菌株被认为代表海栖菌属的一个新物种，为此提出新名称海栖红色菌（Aquimarina rubra sp. nov.）。模式菌株为SS2-9T（=KCTC 52274T=MCCC 1H00142T）。
