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黄芩提取物对小鼠酒精性急性肝损伤具有保护作用，并影响内质网应激机制。

Scutellaria baicalensis Georgi extract protects against alcohol‑induced acute liver injury in mice and affects the mechanism of ER stress.

作者信息

Dong Qingqing, Chu Fei, Wu Chengzhu, Huo Qiang, Gan Huaiyong, Li Xiaoming, Liu Hao

机构信息

Department of Pharmaceutical Preparation, The Second Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.

Department of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.

出版信息

Mol Med Rep. 2016 Apr;13(4):3052-62. doi: 10.3892/mmr.2016.4941. Epub 2016 Feb 23.

DOI:10.3892/mmr.2016.4941
PMID:26936686
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4805105/
Abstract

The aims of the present study were to examine the hepatoprotective effect of Scutellaria baicalensis Georgi extract (Scutellariae Radix extract; SRE) against acute alcohol‑induced liver injury in mice, and investigate the mechanism of endoplasmic reticulum (ER) stress. High performance liquid chromatography was used for the phytochemical analysis of SRE. Animals were administered orally with 50% alcohol (12 ml/kg) 4 h following administration of doses of SRE every day for 14 days, with the exception of normal control group. The protective effect was investigated by measuring the levels of aspartate transaminase (AST), alanine transferase (ALT) and triglyceride (TG) in the serum, and the levels of glutathione (GSH) and malondialdehyde (MDA) in liver tissues. The levels of glucose‑related protein 78 (GRP78) were detected using immunohistochemical localization and an enzyme‑linked immunosorbent assay. Hepatocyte apoptosis was assessed using terminal‑deoxynucleoitidyl transferase mediated nick end labeling. The SRE contained 31.2% baicalin. Pretreatment with SRE had a marked protective effect by reversing the levels of biochemical markers and levels of GRP78 in a dose‑dependent manner. The results of the present study demonstrated that pretreatment with SRE exerted a marked hepatoprotective effect by downregulating the expression of GRP78, which is a marker of ER stress.

摘要

本研究的目的是检测黄芩提取物（黄芩根提取物；SRE）对小鼠急性酒精性肝损伤的肝保护作用，并探讨内质网（ER）应激的机制。采用高效液相色谱法对SRE进行植物化学分析。除正常对照组外，连续14天每天给动物灌胃一定剂量的SRE，4小时后再灌胃50%乙醇（12 ml/kg）。通过检测血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和甘油三酯（TG）的水平，以及肝组织中谷胱甘肽（GSH）和丙二醛（MDA）的水平，研究其保护作用。采用免疫组织化学定位和酶联免疫吸附测定法检测葡萄糖调节蛋白78（GRP78）的水平。采用末端脱氧核苷酸转移酶介导的缺口末端标记法评估肝细胞凋亡。SRE中黄芩苷含量为31.2%。SRE预处理通过剂量依赖性地逆转生化标志物水平和GRP78水平，具有显著的保护作用。本研究结果表明，SRE预处理通过下调ER应激标志物GRP78的表达发挥显著的肝保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/3c3c70d22d8a/MMR-13-04-3052-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/89d86f3e6390/MMR-13-04-3052-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/f71a207cddf0/MMR-13-04-3052-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/3b339d383d09/MMR-13-04-3052-g02.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/82fc920e21bd/MMR-13-04-3052-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/32f61ae140c5/MMR-13-04-3052-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/3c3c70d22d8a/MMR-13-04-3052-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/89d86f3e6390/MMR-13-04-3052-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/f71a207cddf0/MMR-13-04-3052-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/3b339d383d09/MMR-13-04-3052-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/2a3eb00557ef/MMR-13-04-3052-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/82fc920e21bd/MMR-13-04-3052-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/32f61ae140c5/MMR-13-04-3052-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56bd/4805105/3c3c70d22d8a/MMR-13-04-3052-g06.jpg

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