Jia He-Jin, Zhang Peng-Jun, Liu Yu-Lan, Jiang Chao-Guang, Zhu Xu, Tian Ya-Ping
He-Jin Jia, Yu-Lan Liu, Ya-Ping Tian, Core Laboratory of Translational Medicine, State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Beijing 100853, China.
World J Gastroenterol. 2016 Feb 28;22(8):2524-32. doi: 10.3748/wjg.v22.i8.2524.
To investigate the relationship of serum levels of polyunsaturated fatty acid (PUFA) with kinds of cytokines in colorectal cancer (CRC).
Serum samples of 100 CRC patients were collected. The concentration of total n-3 PUFA which included C18:3 n-3, C20:5 n-3, C22:5 n-3, C22:6 n-3 and the total n-6 PUFA included C18:2 n-6, C18:3 n-6, C20:3 n-6, C20:4 n-6, and C22:5 n-6 were detected on GC-2010 Plus Gas Chromatograph with a OmegawaxTM 250 column. Cytokines were detected by MagPlexTM-C microspheres. P values for the trend were estimated by creating a continuous variable using the median value within quartiles.
Interleukin-6 (IL-6) showed significantly positive association with the C20:4 n-6 (P for trend = 0.004). Interferon gamma (IFN-γ) showed significant positive association with the C22:5 n-3 (P for trend = 0.035). IL-8 and matrix metalloproteinase-9 (MMP-9) showed significant inverse association with the C22:6 n-3 (P for trend = 0.049, and 0.021). MMP-2 showed significant inverse association with the C20:5 n-3 (P for trend = 0.008). MMP-7 showed significantly positive association with the ratio of n-6 PUFA and n-3 PUFA (P for trend = 0.008). MMP-7 also showed significantly inverse association with the ratio of C20:4 n-6 and (n-6 PUFA + n-3 PUFA) (P for trend = 0.024). IL-10 (P for trend = 0.023) and IL-6 (P for trend = 0.036) showed significantly positive association with the ratio of C20:4 n-6 and C20:5 n-3.
Our data suggested that serum levels of PUFA is related to the inflammation of CRC, and also play different role in regulation of immune response.
探讨结直肠癌（CRC）患者血清多不饱和脂肪酸（PUFA）水平与多种细胞因子之间的关系。
收集100例CRC患者的血清样本。采用配备OmegawaxTM 250色谱柱的GC-2010 Plus气相色谱仪检测总n-3多不饱和脂肪酸（包括C18:3 n-3、C20:5 n-3、C22:5 n-3、C22:6 n-3）及总n-6多不饱和脂肪酸（包括C18:2 n-6、C18:3 n-6、C20:3 n-6、C20:4 n-6、C22:5 n-6）的浓度。采用MagPlexTM-C微球检测细胞因子。通过使用四分位数内的中位数创建连续变量来估计趋势的P值。
白细胞介素-6（IL-6）与C20:4 n-6呈显著正相关（趋势P值=0.004）。γ干扰素（IFN-γ）与C22:5 n-3呈显著正相关（趋势P值=0.035）。IL-8和基质金属蛋白酶-9（MMP-9）与C22:6 n-3呈显著负相关（趋势P值分别为0.049和0.021）。MMP-2与C20:5 n-3呈显著负相关（趋势P值=0.008）。MMP-7与n-6多不饱和脂肪酸和n-3多不饱和脂肪酸的比值呈显著正相关（趋势P值=0.008）。MMP-7与C20:4 n-6和（n-6多不饱和脂肪酸+n-3多不饱和脂肪酸）的比值也呈显著负相关（趋势P值=0.024）。IL-10（趋势P值=0.023）和IL-6（趋势P值=0.036）与C20:4 n-6和C20:5 n-3的比值呈显著正相关。
我们的数据表明，CRC患者血清PUFA水平与炎症相关，并且在免疫反应调节中发挥不同作用。
