来自补充低浓度烟酸的人微血管内皮细胞的基因表达微阵列数据。
Gene expression microarray data from human microvascular endothelial cells supplemented with a low concentration of niacin.
作者信息
Hughes-Large Jennifer M, Borradaile Nica M
机构信息
Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, Western University, London, Ontario, Canada.
出版信息
Data Brief. 2016 Feb 3;6:899-902. doi: 10.1016/j.dib.2016.01.039. eCollection 2016 Mar.
The systemic lipid modifying drug, niacin, can directly improve human microvascular endothelial cell angiogenic function under lipotoxic conditions, possibly through activation of niacin receptors "Niacin receptor activation improves human microvascular endothelial cell angiogenic function during lipotoxicity" (Hughes-Large et al. 2014). Here we provide accompanying data collected using Affymetrix GeneChip microarrays to identify changes in gene expression in human microvascular endothelial cells treated with 10 μM niacin. Statistical analyses of robust multi-array average (RMA) values revealed that only 16 genes exhibited greater than 1.3-fold differential expression. Of these 16, only 5 were identified protein coding genes, while 3 of the remaining 11 genes appeared to be small nuclear/nucleolar RNAs. Altered expression of EFCAB4B, NAP1L2, and OR13C8 was confirmed by real time quantitative PCR.
全身性脂质调节药物烟酸可在脂毒性条件下直接改善人微血管内皮细胞的血管生成功能，可能是通过激活烟酸受体实现的（“烟酸受体激活改善脂毒性期间人微血管内皮细胞的血管生成功能”，休斯 - 拉格等人，2014年）。在此，我们提供了使用Affymetrix基因芯片微阵列收集的配套数据，以鉴定用10μM烟酸处理的人微血管内皮细胞中基因表达的变化。对稳健多阵列平均值（RMA）值的统计分析表明，只有16个基因表现出大于1.3倍的差异表达。在这16个基因中，只有5个被鉴定为蛋白质编码基因，而其余11个基因中的3个似乎是小核/核仁RNA。通过实时定量PCR证实了EFCAB4B、NAP1L2和OR13C8的表达改变。
Zotero 插件