Li Jian-Ying, Ye Lu-Peng, Che Jia-Qian, Song Jia, You Zheng-Ying, Wang Shao-Hua, Zhong Bo-Xiong
Institute of Developmental and Regenerative Biology, Hangzhou Normal University, Hangzhou 310036, PR China; College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
Data Brief. 2016 Feb 4;6:903-7. doi: 10.1016/j.dib.2016.01.053. eCollection 2016 Mar.
To investigate the functional differentiation among the anterior (A), middle (M), and posterior (P) regions of silkworm middle silk gland (MSG), their proteomes were characterized by shotgun LC-MS/MS analysis with a LTQ-Orbitrap mass spectrometer. To get better proteome identification and quantification, triplicate replicates of mass spectrometry analysis were performed for each sample. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (Vizcaíno et al., 2014) [1] via the PRIDE partner repository (Vizcaino, 2013) [2] with the dataset identifier PXD003371. The peptide identifications that were further processed by PeptideProphet program in Trans-Proteomic Pipeline (TPP) after database search with Mascot software were also available in .XML format files. Data presented here are related to a research article published in Journal of Proteomics by Li et al. (2015) [3].
为了研究家蚕中部丝腺（MSG）前区（A）、中区（M）和后区（P）之间的功能分化，使用LTQ-Orbitrap质谱仪通过鸟枪法LC-MS/MS分析对它们的蛋白质组进行了表征。为了获得更好的蛋白质组鉴定和定量结果，对每个样品进行了三次重复的质谱分析。质谱蛋白质组学数据已通过PRIDE合作伙伴存储库（Vizcaino，2013）[2]存入蛋白质组交换联盟（Vizcaíno等人，2014）[1]，数据集标识符为PXD003371。使用Mascot软件进行数据库搜索后，通过跨蛋白质组学管道（TPP）中的PeptideProphet程序进一步处理的肽段鉴定结果也可以在.XML格式文件中获得。这里呈现的数据与Li等人（2015）[3]发表在《蛋白质组学杂志》上的一篇研究文章相关。
