Khansari Mehdi Rajabnia, Bikloo Shahrzad, Shahreza Sara
Research Center, School of Chemical Engineering, Iran University of Science and Technology, Tehran, Iran.
Research Center, Faculty of Pharmacy, Shaheed Beheshtee University of Medical Sciences, Tehran, Iran.
J Sep Sci. 2016 Mar;39(5):1000-8. doi: 10.1002/jssc.201501178. Epub 2016 Feb 16.
A molecularly imprinted polymer designed for the selective extraction of donepezil from serum samples was synthesized using a noncovalent molecular imprinting approach. The molecularly imprinted polymer was evaluated chromatographically and then its affinity for donepezil was confirmed by solid-phase extraction. The optimal conditions for solid-phase extraction were provided by cartridge conditioning using acidified water purified from a Milli-Q system, sample loading under basic aqueous conditions, clean-up using acetonitrile, and elution with methanol/tetrahydrofuran. Desirable molecular recognition properties of the molecularly imprinted polymer led to good donepezil recoveries (90-102%). The data indicated that the imprinted polymer has a perfect selectivity and affinity for donepezil and could be used for selective extraction and analysis of donepezil in human serum.
采用非共价分子印迹方法合成了一种用于从血清样本中选择性提取多奈哌齐的分子印迹聚合物。对该分子印迹聚合物进行了色谱评估，然后通过固相萃取确认了其对多奈哌齐的亲和力。固相萃取的最佳条件包括：使用从密理博系统纯化的酸化水对小柱进行预处理，在碱性水溶液条件下上样，用乙腈进行净化，并用甲醇/四氢呋喃洗脱。该分子印迹聚合物理想的分子识别特性导致多奈哌齐具有良好的回收率（90 - 102%）。数据表明，该印迹聚合物对多奈哌齐具有完美的选择性和亲和力，可用于人血清中多奈哌齐的选择性提取和分析。
